Characterization of nineteen microsatellite markers for the Japanese clouded salamander, Hynobius nebulosus, using the NGS

Natsuhiko Yoshikawa, Masafumi Matsui, Miho Inoue-Murayama

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The Japanese clouded salamander Hynobius nebulosus, endemic to Western Japan, is ranked as vulnerable in the Red List of Japan. However, microsatellite markers effective for estimating genetic diversity and structures for its conservation measures have never been reported. Therefore, we developed 19 novel microsatellite markers for H. nebulosus. The number of alleles and expected heterozygosity at each locus were 3-14 (mean = 6.63) and 0.22-0.82, respectively, in this species. The markers described here will be useful for investigating the genetic diversity and genetic structure, and planning conservation management of H. nebulosus.

Original languageEnglish
Pages (from-to)603-605
Number of pages3
JournalConservation Genetics Resources
Volume5
Issue number3
DOIs
Publication statusPublished - 2013 Sep 1
Externally publishedYes

Keywords

  • Conservation
  • Genetic diversity
  • Hynobius nebulosus
  • Microsatellite
  • Next-generation sequencing

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

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