Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties

Daisuke Ito, John R. Walker, Charlie S. Thompson, Isabella Moroz, William Lin, Margaret L. Veselits, Antoine M. Hakim, Allen A. Fienberg, Gopal Thinakaran

Research output: Contribution to journalArticle

114 Citations (Scopus)

Abstract

Accumulation of misfolded proteins in the endoplasmic reticulum (ER) induces a highly conserved homeostatic response in all eukaryotic cells, termed the unfolded-protein response (UPR). Here we describe the characterization of stanniocalcin 2 (STC2), a mammalian homologue of a calcium- and phosphate-regulating hormone first identified in fish, as a novel target of the UPR. Expression of STC2 gene is rapidly upregulated in cultured cells after exposure to tunicamycin and thapsigargin, by ATF4 after activation of the ER-resident kinase PERK. In addition, STC2 expression is also activated in neuronal cells by oxidative stress and hypoxia but not by several cellular stresses unrelated to the UPR. In contrast, expression of another homologue, STC1, is only upregulated by hypoxia independent of PERK or ATF4 expression. In vivo studies revealed that rat cortical neurons rapidly upregulate STC2 after transient middle cerebral artery occlusion. Finally, siRNA-mediated inhibition of STC2 expression renders N2a neuroblastoma cells and HeLa cells significantly more vulnerable to apoptotic cell death after treatment with thapsigargin, and overexpression of STC2 attenuated thapsigargin-induced cell death. Consequently, induced STC2 expression is an essential feature of survival component of the UPR.

Original languageEnglish
Pages (from-to)9456-9469
Number of pages14
JournalMolecular and Cellular Biology
Volume24
Issue number21
DOIs
Publication statusPublished - 2004 Nov
Externally publishedYes

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Unfolded Protein Response
Thapsigargin
Endoplasmic Reticulum
Cell Death
Tunicamycin
Middle Cerebral Artery Infarction
Eukaryotic Cells
teleocalcin
Neuroblastoma
HeLa Cells
Small Interfering RNA
Cultured Cells
Fishes
Oxidative Stress
Up-Regulation
Hormones
Neurons

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties. / Ito, Daisuke; Walker, John R.; Thompson, Charlie S.; Moroz, Isabella; Lin, William; Veselits, Margaret L.; Hakim, Antoine M.; Fienberg, Allen A.; Thinakaran, Gopal.

In: Molecular and Cellular Biology, Vol. 24, No. 21, 11.2004, p. 9456-9469.

Research output: Contribution to journalArticle

Ito, D, Walker, JR, Thompson, CS, Moroz, I, Lin, W, Veselits, ML, Hakim, AM, Fienberg, AA & Thinakaran, G 2004, 'Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties', Molecular and Cellular Biology, vol. 24, no. 21, pp. 9456-9469. https://doi.org/10.1128/MCB.24.21.9456-9469.2004
Ito, Daisuke ; Walker, John R. ; Thompson, Charlie S. ; Moroz, Isabella ; Lin, William ; Veselits, Margaret L. ; Hakim, Antoine M. ; Fienberg, Allen A. ; Thinakaran, Gopal. / Characterization of stanniocalcin 2, a novel target of the mammalian unfolded protein response with cytoprotective properties. In: Molecular and Cellular Biology. 2004 ; Vol. 24, No. 21. pp. 9456-9469.
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