Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene

Y. Ogawa, Hiroshi Itoh, O. Nakagawa, G. Shirakami, N. Tamura, T. Yoshimasa, K. Nagata, N. Yoshida, K. Nakao

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Brain natriuretic peptide (BNP) is a cardiac hormone that occurs predominantly in the ventricle, and synthesis and secretion of BNP are greatly augmented in patients with congestive heart failure and in animal models of ventricular hypertrophy. In order to elucidate the molecular mechanisms underlying the human BNP gene expression in the heart, the human BNP gene was isolated from a size-selected genomic minilibrary. The 1.9-kb human BNP 5′-flanking region (-1813 to +110) contained an array of putative cis-acting regulatory elements. Various lengths of the cloned 5′-flanking sequences were linked upstream to the bacterial chloramphenicol acetyltransferase (CAT) gene, and their promoter activities were assayed. The 1.9-kb promoter region showed a high-level CAT activity in cultured neonatal rat ventricular cardiocytes. When the CT-rich sequences (-1288 to -1095) were deleted, the high-level activity was reduced to approximately 30%. The 399-bp BNP 5′ flanking region (-289 to +110) showed approximately 10% activity of the 1.9-kb region. Furthermore, using human-rodent somatic hybrid cell lines, the BNP gene was assigned to human chromosome 1, on which the atrial natriuretic peptide gene is localized. The present study leads to a better understanding of the molecular mechanisms for the human BNP gene expression in the heart.

Original languageEnglish
Pages (from-to)457-463
Number of pages7
JournalJournal of Molecular Medicine
Volume73
Issue number9
DOIs
Publication statusPublished - 1995 Sep
Externally publishedYes

Fingerprint

5' Flanking Region
Brain Natriuretic Peptide
Genes
Chloramphenicol O-Acetyltransferase
Gene Expression
Chromosomes, Human, Pair 1
Human Chromosomes
Atrial Natriuretic Factor
Genetic Promoter Regions
Hypertrophy
Rodentia
Animal Models
Heart Failure
Hormones
Cell Line

Keywords

  • Atrial natriuretic peptide
  • Brain natriuretic peptide
  • Cardiac hormone
  • Chromosomal assignment

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene. / Ogawa, Y.; Itoh, Hiroshi; Nakagawa, O.; Shirakami, G.; Tamura, N.; Yoshimasa, T.; Nagata, K.; Yoshida, N.; Nakao, K.

In: Journal of Molecular Medicine, Vol. 73, No. 9, 09.1995, p. 457-463.

Research output: Contribution to journalArticle

Ogawa, Y, Itoh, H, Nakagawa, O, Shirakami, G, Tamura, N, Yoshimasa, T, Nagata, K, Yoshida, N & Nakao, K 1995, 'Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene', Journal of Molecular Medicine, vol. 73, no. 9, pp. 457-463. https://doi.org/10.1007/BF00202264
Ogawa, Y. ; Itoh, Hiroshi ; Nakagawa, O. ; Shirakami, G. ; Tamura, N. ; Yoshimasa, T. ; Nagata, K. ; Yoshida, N. ; Nakao, K. / Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene. In: Journal of Molecular Medicine. 1995 ; Vol. 73, No. 9. pp. 457-463.
@article{c8705c9c40dd435c97df99f7c844badd,
title = "Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene",
abstract = "Brain natriuretic peptide (BNP) is a cardiac hormone that occurs predominantly in the ventricle, and synthesis and secretion of BNP are greatly augmented in patients with congestive heart failure and in animal models of ventricular hypertrophy. In order to elucidate the molecular mechanisms underlying the human BNP gene expression in the heart, the human BNP gene was isolated from a size-selected genomic minilibrary. The 1.9-kb human BNP 5′-flanking region (-1813 to +110) contained an array of putative cis-acting regulatory elements. Various lengths of the cloned 5′-flanking sequences were linked upstream to the bacterial chloramphenicol acetyltransferase (CAT) gene, and their promoter activities were assayed. The 1.9-kb promoter region showed a high-level CAT activity in cultured neonatal rat ventricular cardiocytes. When the CT-rich sequences (-1288 to -1095) were deleted, the high-level activity was reduced to approximately 30{\%}. The 399-bp BNP 5′ flanking region (-289 to +110) showed approximately 10{\%} activity of the 1.9-kb region. Furthermore, using human-rodent somatic hybrid cell lines, the BNP gene was assigned to human chromosome 1, on which the atrial natriuretic peptide gene is localized. The present study leads to a better understanding of the molecular mechanisms for the human BNP gene expression in the heart.",
keywords = "Atrial natriuretic peptide, Brain natriuretic peptide, Cardiac hormone, Chromosomal assignment",
author = "Y. Ogawa and Hiroshi Itoh and O. Nakagawa and G. Shirakami and N. Tamura and T. Yoshimasa and K. Nagata and N. Yoshida and K. Nakao",
year = "1995",
month = "9",
doi = "10.1007/BF00202264",
language = "English",
volume = "73",
pages = "457--463",
journal = "Journal of Molecular Medicine",
issn = "0946-2716",
publisher = "Springer Verlag",
number = "9",

}

TY - JOUR

T1 - Characterization of the 5′-flanking region and chromosomal assignment of the human brain natriuretic peptide gene

AU - Ogawa, Y.

AU - Itoh, Hiroshi

AU - Nakagawa, O.

AU - Shirakami, G.

AU - Tamura, N.

AU - Yoshimasa, T.

AU - Nagata, K.

AU - Yoshida, N.

AU - Nakao, K.

PY - 1995/9

Y1 - 1995/9

N2 - Brain natriuretic peptide (BNP) is a cardiac hormone that occurs predominantly in the ventricle, and synthesis and secretion of BNP are greatly augmented in patients with congestive heart failure and in animal models of ventricular hypertrophy. In order to elucidate the molecular mechanisms underlying the human BNP gene expression in the heart, the human BNP gene was isolated from a size-selected genomic minilibrary. The 1.9-kb human BNP 5′-flanking region (-1813 to +110) contained an array of putative cis-acting regulatory elements. Various lengths of the cloned 5′-flanking sequences were linked upstream to the bacterial chloramphenicol acetyltransferase (CAT) gene, and their promoter activities were assayed. The 1.9-kb promoter region showed a high-level CAT activity in cultured neonatal rat ventricular cardiocytes. When the CT-rich sequences (-1288 to -1095) were deleted, the high-level activity was reduced to approximately 30%. The 399-bp BNP 5′ flanking region (-289 to +110) showed approximately 10% activity of the 1.9-kb region. Furthermore, using human-rodent somatic hybrid cell lines, the BNP gene was assigned to human chromosome 1, on which the atrial natriuretic peptide gene is localized. The present study leads to a better understanding of the molecular mechanisms for the human BNP gene expression in the heart.

AB - Brain natriuretic peptide (BNP) is a cardiac hormone that occurs predominantly in the ventricle, and synthesis and secretion of BNP are greatly augmented in patients with congestive heart failure and in animal models of ventricular hypertrophy. In order to elucidate the molecular mechanisms underlying the human BNP gene expression in the heart, the human BNP gene was isolated from a size-selected genomic minilibrary. The 1.9-kb human BNP 5′-flanking region (-1813 to +110) contained an array of putative cis-acting regulatory elements. Various lengths of the cloned 5′-flanking sequences were linked upstream to the bacterial chloramphenicol acetyltransferase (CAT) gene, and their promoter activities were assayed. The 1.9-kb promoter region showed a high-level CAT activity in cultured neonatal rat ventricular cardiocytes. When the CT-rich sequences (-1288 to -1095) were deleted, the high-level activity was reduced to approximately 30%. The 399-bp BNP 5′ flanking region (-289 to +110) showed approximately 10% activity of the 1.9-kb region. Furthermore, using human-rodent somatic hybrid cell lines, the BNP gene was assigned to human chromosome 1, on which the atrial natriuretic peptide gene is localized. The present study leads to a better understanding of the molecular mechanisms for the human BNP gene expression in the heart.

KW - Atrial natriuretic peptide

KW - Brain natriuretic peptide

KW - Cardiac hormone

KW - Chromosomal assignment

UR - http://www.scopus.com/inward/record.url?scp=0028885640&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028885640&partnerID=8YFLogxK

U2 - 10.1007/BF00202264

DO - 10.1007/BF00202264

M3 - Article

C2 - 8528749

AN - SCOPUS:0028885640

VL - 73

SP - 457

EP - 463

JO - Journal of Molecular Medicine

JF - Journal of Molecular Medicine

SN - 0946-2716

IS - 9

ER -