Characterization of the 5′-noncoding region of the mouse choline acetyltransferase gene in transgenic mice

J. M. Naciff, M. M. Behbehani, Hidemi Misawa, J. R. Dedman

Research output: Contribution to journalArticle

Abstract

Choline acetyltransferase (ChAT) is a specific phenotypic marker of cholinergic neurons. Previous reports showed that different upstream regions of the ChAT gene are necessary for cell-type specific expression of reporter genes in cholinergic cell lines. The identity of the mouse ChAT promoter region controlling the establishment, maintenance, and plasticity of the cholinergic phenotype in vivo is not known. We characterized the promoter region of the mouse ChAT gene in transgenic mice, using β-galactosidase (β-gal) as a reporter. A 3401-bp segment from the 5′-untranslated region of the mouse ChAT gene was sufficient to direct the expression of β-gal to selected neurons of the nervous system (NS); it did not provide cholinergic specificity A larger fragment (∼6 Kb) of this region encompassing most of the coding sequence for the putative mouse vesicular acetylcholine transporter (VAChT) contained within the first intron of the ChAT gene, directs correct developmental and tissue-specific expression to cholinergic cells in transgenic mice. This fragment targeted β-gal expression only to cholinergic regions of the NS of transgenic mice, including basal forebrain, striatum, cortex, brain stem, and exclusively to α-motor neurons in the ventral horn of the spinal cord. This ChAT promoter will allow targeting of specific gene products to cholinergic neurons, in order to develop animal models for human diseases where injury and degeneration of chotinergic neurons is involved.

Original languageEnglish
JournalFASEB Journal
Volume11
Issue number3
Publication statusPublished - 1997
Externally publishedYes

Fingerprint

choline acetyltransferase
Choline O-Acetyltransferase
cholinergic agents
Cholinergic Agents
Transgenic Mice
Genes
genetically modified organisms
Neurons
mice
neurons
genes
Cholinergic Neurons
promoter regions
Neurology
Genetic Promoter Regions
nervous system
Nervous System
Vesicular Acetylcholine Transport Proteins
Galactosidases
galactosidases

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Characterization of the 5′-noncoding region of the mouse choline acetyltransferase gene in transgenic mice. / Naciff, J. M.; Behbehani, M. M.; Misawa, Hidemi; Dedman, J. R.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

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