Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803

Kei Nanatani, Toshiaki Shijuku, Masaro Akai, Yoshinori Yukutake, Masato Yasui, Shin Hamamoto, Kiyoshi Onai, Megumi Morishita, Masahiro Ishiura, Nobuyuki Uozumi

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that ΔsymscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.

Original languageEnglish
Pages (from-to)238-242
Number of pages5
JournalChannels
Volume7
Issue number4
DOIs
Publication statusPublished - 2013

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Synechocystis
Osmotic Pressure
Gene encoding
Spectrophotometry
Cell membranes
Monitoring
Cell Size
Cell Membrane
Genes

Keywords

  • Circadian rhythm
  • Hypoosmotic stress adaptation
  • Mechanosensitive channel
  • Synechocystis

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Medicine(all)

Cite this

Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803. / Nanatani, Kei; Shijuku, Toshiaki; Akai, Masaro; Yukutake, Yoshinori; Yasui, Masato; Hamamoto, Shin; Onai, Kiyoshi; Morishita, Megumi; Ishiura, Masahiro; Uozumi, Nobuyuki.

In: Channels, Vol. 7, No. 4, 2013, p. 238-242.

Research output: Contribution to journalArticle

Nanatani, K, Shijuku, T, Akai, M, Yukutake, Y, Yasui, M, Hamamoto, S, Onai, K, Morishita, M, Ishiura, M & Uozumi, N 2013, 'Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803', Channels, vol. 7, no. 4, pp. 238-242. https://doi.org/10.4161/chan.25350
Nanatani, Kei ; Shijuku, Toshiaki ; Akai, Masaro ; Yukutake, Yoshinori ; Yasui, Masato ; Hamamoto, Shin ; Onai, Kiyoshi ; Morishita, Megumi ; Ishiura, Masahiro ; Uozumi, Nobuyuki. / Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803. In: Channels. 2013 ; Vol. 7, No. 4. pp. 238-242.
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AU - Akai, Masaro

AU - Yukutake, Yoshinori

AU - Yasui, Masato

AU - Hamamoto, Shin

AU - Onai, Kiyoshi

AU - Morishita, Megumi

AU - Ishiura, Masahiro

AU - Uozumi, Nobuyuki

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AB - Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that ΔsymscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.

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