Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803

Kei Nanatani; Toshiaki Shijuku; Masaro Akai; Yoshinori Yukutake; Masato Yasui; Shin Hamamoto; Kiyoshi Onai; Megumi Morishita; Masahiro Ishiura; Nobuyuki Uozumi

doi:10.4161/chan.25350

Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803

Kei Nanatani, Toshiaki Shijuku, Masaro Akai, Yoshinori Yukutake, Masato Yasui, Shin Hamamoto, Kiyoshi Onai, Megumi Morishita, Masahiro Ishiura, Nobuyuki Uozumi

Department of Pharmacology

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

Abstract

Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that ΔsymscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.

Original language	English
Pages (from-to)	238-242
Number of pages	5
Journal	Channels
Volume	7
Issue number	4
DOIs	https://doi.org/10.4161/chan.25350
Publication status	Published - 2013

Keywords

Circadian rhythm
Hypoosmotic stress adaptation
Mechanosensitive channel
Synechocystis

ASJC Scopus subject areas

Biophysics
Biochemistry

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10.4161/chan.25350

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@article{df84909b67974247bef6ad144f107f54,

title = "Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803",

abstract = "Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that ΔsymscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.",

keywords = "Circadian rhythm, Hypoosmotic stress adaptation, Mechanosensitive channel, Synechocystis",

author = "Kei Nanatani and Toshiaki Shijuku and Masaro Akai and Yoshinori Yukutake and Masato Yasui and Shin Hamamoto and Kiyoshi Onai and Megumi Morishita and Masahiro Ishiura and Nobuyuki Uozumi",

note = "Funding Information: We thank Tatsuo Omata (Nagoya University, Japan) for generously supplying antibodies for NrtA, Teruo Ogawa (Nagoya University, Japan) and Eva-Mari Aro (University of Turku, Finland) for antibodies for NdhD3 and NdhF3 and Anke Reinders (University of Minnesota) for critical reading of the manuscript. This work was supported by JSPS (22380056, 24246045, 24580100, 24658090 and 25292055).",

year = "2013",

doi = "10.4161/chan.25350",

language = "English",

volume = "7",

pages = "238--242",

journal = "Channels",

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publisher = "Landes Bioscience",

number = "4",

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TY - JOUR

T1 - Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803

AU - Nanatani, Kei

AU - Shijuku, Toshiaki

AU - Akai, Masaro

AU - Yukutake, Yoshinori

AU - Yasui, Masato

AU - Hamamoto, Shin

AU - Onai, Kiyoshi

AU - Morishita, Megumi

AU - Ishiura, Masahiro

AU - Uozumi, Nobuyuki

N1 - Funding Information: We thank Tatsuo Omata (Nagoya University, Japan) for generously supplying antibodies for NrtA, Teruo Ogawa (Nagoya University, Japan) and Eva-Mari Aro (University of Turku, Finland) for antibodies for NdhD3 and NdhF3 and Anke Reinders (University of Minnesota) for critical reading of the manuscript. This work was supported by JSPS (22380056, 24246045, 24580100, 24658090 and 25292055).

PY - 2013

Y1 - 2013

N2 - Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that ΔsymscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.

AB - Synechocystis sp strain PCC 6803 contains one gene encoding a putative large conductance mechanosensitive channel homolog [named SyMscL (slr0875)]. However, it is unclear whether SyMscL contributes to the adaptation to hypoosmotic stress in Synechocystis. Here we report the in vivo characteristics of SyMscL. SyMscL was mainly expressed in the plasma membrane of Synechocystis. Cell volume monitoring using stopped-flow spectrophotometry showed that ΔsymscL cells swelled more rapidly than wild-type cells under hypoosmotic stress conditions. Expression of symscL was under circadian control, and its peak corresponded to the beginning of subjective night. These results indicate that SyMscL functioned as one component of the osmotic homeostatic regulatory system of the cell coordinating the response of Synechocystis to daily metabolic osmotic fluctuations and environmental changes.

KW - Circadian rhythm

KW - Hypoosmotic stress adaptation

KW - Mechanosensitive channel

KW - Synechocystis

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Characterization of the role of a mechanosensitive channel in osmotic down shock adaptation in Synechocystis sp PCC 6803

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