TY - JOUR
T1 - Characterization of the Su Antigen, a Macromolecular Complex of 100/102 and 200-kDa Proteins Recognized by Autoantibodies in Systemic Rheumatic Diseases
AU - Satoh, Minoru
AU - Langdon, Jenifer J.
AU - Chou, Chih Hao
AU - McCauliffe, Daniel P.
AU - Treadwell, Edward L.
AU - Ogasawara, Takashi
AU - Hirakata, Michito
AU - Suwa, Akira
AU - Cohen, Philip L.
AU - Eisenberg, Robert A.
AU - Reeves, Westley H.
N1 - Copyright:
Copyright 2017 Elsevier B.V., All rights reserved.
PY - 1994/10
Y1 - 1994/10
N2 - The Su autoantigen was characterized biochemically using human and murine autoimmune sera and the clinical significance of anti-Su antibodies was studied in 236 Japanese and 160 American patients with systemic rheumatic diseases. Anti-Su in immunodiffusion (ID) was strongly associated with immunoprecipitation of one or more 100- to 102-kDa proteins by MRL/lpr mouse sera (27/32 of ID positive vs 4/20 of ID negative, P = 0.000016), and all four human anti-Su reference sera immunoprecipitated the 100/102-kDa protein(s). In addition, all sera immunoprecipitated a less efficiently labeled ∼200-kDa protein that comigrated on sucrose density gradients with the 100/102-kDa proteins. Based on these data, a complex of the 100/102-kDa and 200-kDa proteins is likely to be the main target of anti-Su antibodies. Three of four anti-Su monospecific sera were negative for immunofluorescent antinuclear antibodies (ANA), suggesting anti-Su antibodies may be associated with a negative ANA in some cases. Autoantibodies to Su were detected frequently by immunoprecipitation in systemic lupus erythematosus (17-21%), scleroderma (13-20%), and overlap syndrome (22-40%) and were associated with autoantibodies to Ku.
AB - The Su autoantigen was characterized biochemically using human and murine autoimmune sera and the clinical significance of anti-Su antibodies was studied in 236 Japanese and 160 American patients with systemic rheumatic diseases. Anti-Su in immunodiffusion (ID) was strongly associated with immunoprecipitation of one or more 100- to 102-kDa proteins by MRL/lpr mouse sera (27/32 of ID positive vs 4/20 of ID negative, P = 0.000016), and all four human anti-Su reference sera immunoprecipitated the 100/102-kDa protein(s). In addition, all sera immunoprecipitated a less efficiently labeled ∼200-kDa protein that comigrated on sucrose density gradients with the 100/102-kDa proteins. Based on these data, a complex of the 100/102-kDa and 200-kDa proteins is likely to be the main target of anti-Su antibodies. Three of four anti-Su monospecific sera were negative for immunofluorescent antinuclear antibodies (ANA), suggesting anti-Su antibodies may be associated with a negative ANA in some cases. Autoantibodies to Su were detected frequently by immunoprecipitation in systemic lupus erythematosus (17-21%), scleroderma (13-20%), and overlap syndrome (22-40%) and were associated with autoantibodies to Ku.
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U2 - 10.1006/clin.1994.1179
DO - 10.1006/clin.1994.1179
M3 - Article
C2 - 7923910
AN - SCOPUS:0028109277
SN - 1521-6616
VL - 73
SP - 132
EP - 141
JO - Clinical Immunology
JF - Clinical Immunology
IS - 1
ER -
