Characterization of the Su Antigen, a Macromolecular Complex of 100/102 and 200-kDa Proteins Recognized by Autoantibodies in Systemic Rheumatic Diseases

Minoru Satoh, Jenifer J. Langdon, Chih Hao Chou, Daniel P. McCauliffe, Edward L. Treadwell, Takashi Ogasawara, Michito Hirakata, Akira Suwa, Philip L. Cohen, Robert A. Eisenberg, Westley H. Reeves

Research output: Contribution to journalArticle

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Abstract

The Su autoantigen was characterized biochemically using human and murine autoimmune sera and the clinical significance of anti-Su antibodies was studied in 236 Japanese and 160 American patients with systemic rheumatic diseases. Anti-Su in immunodiffusion (ID) was strongly associated with immunoprecipitation of one or more 100- to 102-kDa proteins by MRL/lpr mouse sera (27/32 of ID positive vs 4/20 of ID negative, P = 0.000016), and all four human anti-Su reference sera immunoprecipitated the 100/102-kDa protein(s). In addition, all sera immunoprecipitated a less efficiently labeled ∼200-kDa protein that comigrated on sucrose density gradients with the 100/102-kDa proteins. Based on these data, a complex of the 100/102-kDa and 200-kDa proteins is likely to be the main target of anti-Su antibodies. Three of four anti-Su monospecific sera were negative for immunofluorescent antinuclear antibodies (ANA), suggesting anti-Su antibodies may be associated with a negative ANA in some cases. Autoantibodies to Su were detected frequently by immunoprecipitation in systemic lupus erythematosus (17-21%), scleroderma (13-20%), and overlap syndrome (22-40%) and were associated with autoantibodies to Ku.

Original languageEnglish
Pages (from-to)132-141
Number of pages10
JournalClinical Immunology and Immunopathology
Volume73
Issue number1
DOIs
Publication statusPublished - 1994 Oct
Externally publishedYes

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Macromolecular Substances
Rheumatic Diseases
Autoantibodies
Immunodiffusion
Anti-Idiotypic Antibodies
Serum
Antinuclear Antibodies
Proteins
Immunoprecipitation
Inbred MRL lpr Mouse
Systemic Lupus Erythematosus
Sucrose
Su autoantigen

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Immunology and Allergy
  • Immunology

Cite this

Characterization of the Su Antigen, a Macromolecular Complex of 100/102 and 200-kDa Proteins Recognized by Autoantibodies in Systemic Rheumatic Diseases. / Satoh, Minoru; Langdon, Jenifer J.; Chou, Chih Hao; McCauliffe, Daniel P.; Treadwell, Edward L.; Ogasawara, Takashi; Hirakata, Michito; Suwa, Akira; Cohen, Philip L.; Eisenberg, Robert A.; Reeves, Westley H.

In: Clinical Immunology and Immunopathology, Vol. 73, No. 1, 10.1994, p. 132-141.

Research output: Contribution to journalArticle

Satoh, M, Langdon, JJ, Chou, CH, McCauliffe, DP, Treadwell, EL, Ogasawara, T, Hirakata, M, Suwa, A, Cohen, PL, Eisenberg, RA & Reeves, WH 1994, 'Characterization of the Su Antigen, a Macromolecular Complex of 100/102 and 200-kDa Proteins Recognized by Autoantibodies in Systemic Rheumatic Diseases', Clinical Immunology and Immunopathology, vol. 73, no. 1, pp. 132-141. https://doi.org/10.1006/clin.1994.1179
Satoh, Minoru ; Langdon, Jenifer J. ; Chou, Chih Hao ; McCauliffe, Daniel P. ; Treadwell, Edward L. ; Ogasawara, Takashi ; Hirakata, Michito ; Suwa, Akira ; Cohen, Philip L. ; Eisenberg, Robert A. ; Reeves, Westley H. / Characterization of the Su Antigen, a Macromolecular Complex of 100/102 and 200-kDa Proteins Recognized by Autoantibodies in Systemic Rheumatic Diseases. In: Clinical Immunology and Immunopathology. 1994 ; Vol. 73, No. 1. pp. 132-141.
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