Characterization of two novel polysaccharides having immunological activities from the root of panax ginseng.

Masashi Tomoda, Keiko Hirabayashi, Noriko Shimizu, Ryōko Gonda, Naoko Ōhara, Katsutoshi Takada

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Two acidic polysaccharides, called ginsenan-S-IA and ginsenan S-IIA, were isolated from the root of Panax ginseng C. A. MEYER. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 5.6×104 and 1.0×105, respectively. Ginsenan S-IA is composed of L-arabinose : D-galactose : D-galacturonic acid in the molar ratio of 8 : 8 : 1, and ginsenan S-IIA is composed of L-arabinose : D-galactose : D-glucose : D-galacturonic acid in the molar ratio of 15 : 10 : 2 : 5, in addition to small amounts of O-acetyl groups. About a half (ginsenan S-IA) and about a quarter (ginsenan S-IIA) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and periodate oxidation studies indicated that their structural features include mainly α-1, 5-linked L-arabino-β-3, 6-branched D-galactan type structural units. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test and pronounced anti-complementary activity. These substances are the first examples having a relatively high content of both α-3, 5-branched L-arabinose and β-1, 4-linked D-galactose units among the acidic arabinogalactans with activities on phagocytosis and anti-complement.

Original languageEnglish
Pages (from-to)1087-1090
Number of pages4
JournalBiological and Pharmaceutical Bulletin
Issue number11
Publication statusPublished - 1993 Jan 1



  • Panax ginseng
  • acidic arabinogalactan
  • ginsenan S-IA
  • ginsenan S-IIA
  • immunological activity
  • polysaccharide structure

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science

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