Characterization of Two Polysaccharides Having Activity on the Reticuloendothelial System from the Root of Rehmannia glutinosa

Masashi Tomoda, Hiroko Miyamoto, Noriko Shimizu, Rvoko Gonda, Naoko Ohara

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Two acidic polysaccharides, called rehmannan SA and rehmannan SB, were isolated from the dried root of Rehmannia glutinosa Liboschitz. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 6.4 x 104 and 7.9 x 104, respectively. They were commonly composed of L-arabinose: D-galactose: L-rhamnose: D-galacturonic acid in the molar ratios of 10:10:1:1 (rehmannan SA) and 14:7:3:8 (rehmannan SB), in addition to small amounts of peptide moieties. About eighty percent (rehmannan SA) and about thirty percent (rehmannan SB) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and the controlled Smith degradation studies indicated the structural features of rehmannan SB, the major one in terms of the yield. It has a core structural unit of a characteristic rhamnose-rich arabino-3,6-galactan type. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume42
Issue number3
DOIs
Publication statusPublished - 1994 Jan 1

Keywords

  • Rehmannia glutinosa
  • acidic arabinogalactan
  • immunological activity
  • polysaccharide structure
  • rehmannan SB

ASJC Scopus subject areas

  • Chemistry(all)
  • Drug Discovery

Fingerprint Dive into the research topics of 'Characterization of Two Polysaccharides Having Activity on the Reticuloendothelial System from the Root of Rehmannia glutinosa'. Together they form a unique fingerprint.

  • Cite this