Characterization of two polysaccharides having activity on the reticuloendothelial system from the root of Rehmannia glutinosa

M. Tomoda, H. Miyamoto, N. Shimizu, Ryoko Gonda, N. Ohara

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18 Citations (Scopus)

Abstract

Two acidic polysaccharides, called rehmannan SA and rehmannan SB, were isolated from the dried root of Rehmannia glutinosa LIBOSCHITZ. They were homogeneous on electrophoresis and gel chromatography, and their molecular masses were estimated to be 6.4 x 104 and 7.9 x 104, respectively. They were commonly composed of L-arabinose:D-galactose:L-rhamnose:D-galacturonic acid in the molar ratios of 10:10:1:1 (rehmannan SA) and 14:7:3:8 (rehmannan SB), in addition to small amounts of peptide moieties. About eighty percent (rehmannan SA) and about thirty percent (rehmannan SB) of the hexuronic acid residues exist as methyl esters. Reduction of carboxyl groups, methylation analysis, nuclear magnetic resonance and the controlled Smith degradation studies indicated the structural features of rehmannan SB, the major one in terms of the yield. It has a core structural unit of a characteristic rhamnose-rich arabino-3,6-galactan type. Both polysaccharides showed remarkable reticuloendothelial system-potentiating activity in a carbon clearance test.

Original languageEnglish
Pages (from-to)625-629
Number of pages5
JournalChemical and Pharmaceutical Bulletin
Volume42
Issue number3
Publication statusPublished - 1994

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Rehmannia
Rhamnose
Mononuclear Phagocyte System
Polysaccharides
Hexuronic Acids
Galactans
Arabinose
Methylation
Molecular mass
Chromatography
Electrophoresis
Galactose
Gel Chromatography
Esters
Magnetic Resonance Spectroscopy
Carbon
Gels
Nuclear magnetic resonance
Degradation
Peptides

ASJC Scopus subject areas

  • Chemistry(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmacology

Cite this

Characterization of two polysaccharides having activity on the reticuloendothelial system from the root of Rehmannia glutinosa. / Tomoda, M.; Miyamoto, H.; Shimizu, N.; Gonda, Ryoko; Ohara, N.

In: Chemical and Pharmaceutical Bulletin, Vol. 42, No. 3, 1994, p. 625-629.

Research output: Contribution to journalArticle

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