Chemical biology of compounds obtained from screening using disease models

Etsu Tashiro, Masaya Imoto

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Bioactive compounds are extremely powerful tools for studying biological systems because they can rapidly, conditionally, often reversibly, and dose-dependently modulate the biological function of living cells. Moreover, they are expected to be drug seeds for chemotherapy of several diseases. Two approaches are used to find and obtain bioactive compounds, namely, molecular-target-based screening and phenotypic screening. Through phenotypic screening that mimics tumor metastasis, multi-drug resistance, and Parkinson's disease, we identified several compounds that inhibit cancer cell migration, anti-apoptotic function of Bcl-2/Bcl-xL, and neuronal cell death. By using MEK inhibitor that was developed by target-based screening, we discovered that MEK inhibitor selectively induces apoptosis in tumor cells with β-catenin mutation. Using target-based screening, we identified arabilin, a novel androgen antagonist. In this review, we introduce our recent studies on the identification of bioactive compounds by phenotypic screening and by target-based screening for drug-seed discovery.

Original languageEnglish
Article number633
Pages (from-to)1651-1660
Number of pages10
JournalArchives of Pharmacal Research
Volume38
Issue number9
DOIs
Publication statusPublished - 2015 Sep 1

Keywords

  • Androgen
  • Apoptosis
  • Cell migration
  • MEK
  • Parkinson's disease
  • β-Catenin

ASJC Scopus subject areas

  • Drug Discovery
  • Molecular Medicine
  • Organic Chemistry

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