Chemical decontamination of iPS cell-derived neural cell mixtures

Di Mao; Xie Khim Watson Chung; Tomoko Andoh-Noda; Ying Qin; Shin Ichi Sato; Yasushi Takemoto; Wado Akamatsu; Hideyuki Okano; Motonari Uesugi

doi:10.1039/c7cc08686e

Chemical decontamination of iPS cell-derived neural cell mixtures

Di Mao, Xie Khim Watson Chung, Tomoko Andoh-Noda, Ying Qin, Shin Ichi Sato, Yasushi Takemoto, Wado Akamatsu, Hideyuki Okano, Motonari Uesugi

Department of Physiology

Research output: Contribution to journal › Article › peer-review

5 Citations (Scopus)

Abstract

This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.

Original language	English
Pages (from-to)	1355-1358
Number of pages	4
Journal	Chemical Communications
Volume	54
Issue number	11
DOIs	https://doi.org/10.1039/c7cc08686e
Publication status	Published - 2018

ASJC Scopus subject areas

Catalysis
Electronic, Optical and Magnetic Materials
Ceramics and Composites
Chemistry(all)
Surfaces, Coatings and Films
Metals and Alloys
Materials Chemistry

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10.1039/c7cc08686e

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title = "Chemical decontamination of iPS cell-derived neural cell mixtures",

abstract = "This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.",

author = "Di Mao and Chung, {Xie Khim Watson} and Tomoko Andoh-Noda and Ying Qin and Sato, {Shin Ichi} and Yasushi Takemoto and Wado Akamatsu and Hideyuki Okano and Motonari Uesugi",

note = "Funding Information: This research is supported by the project, {\textquoteleft}{\textquoteleft}Development of Cell Production and Processing Systems for Commercialization of Regenerative Medicine,{\textquoteright}{\textquoteright} from the Japan Agency for Medical Research and Development, AMED. This work is also supported in part by JSPS (26220206 to M.U.). This work was inspired by JSPS CORE-to-CORE {\textquoteleft}{\textquoteleft}Asian Chemical Biology Initiative.{\textquoteright}{\textquoteright} Publisher Copyright: {\textcopyright} 2018 The Royal Society of Chemistry.",

year = "2018",

doi = "10.1039/c7cc08686e",

language = "English",

volume = "54",

pages = "1355--1358",

journal = "Chemical Communications",

issn = "1359-7345",

publisher = "Royal Society of Chemistry",

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TY - JOUR

T1 - Chemical decontamination of iPS cell-derived neural cell mixtures

AU - Mao, Di

AU - Chung, Xie Khim Watson

AU - Andoh-Noda, Tomoko

AU - Qin, Ying

AU - Sato, Shin Ichi

AU - Takemoto, Yasushi

AU - Akamatsu, Wado

AU - Okano, Hideyuki

AU - Uesugi, Motonari

N1 - Funding Information: This research is supported by the project, ‘‘Development of Cell Production and Processing Systems for Commercialization of Regenerative Medicine,’’ from the Japan Agency for Medical Research and Development, AMED. This work is also supported in part by JSPS (26220206 to M.U.). This work was inspired by JSPS CORE-to-CORE ‘‘Asian Chemical Biology Initiative.’’ Publisher Copyright: © 2018 The Royal Society of Chemistry.

PY - 2018

Y1 - 2018

N2 - This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.

AB - This report describes the design and evaluation of phosphorylated 7-ethyl-10-hydroxycamptothecin (SN38-P), which selectively eliminates tumor-forming proliferative stem cells, including human induced pluripotent stem cells (hiPSCs) and neural stem cells, from iPSC-derived neural cell mixtures. Results of the present study demonstrate that simple phosphorylation of an anticancer drug can provide a safe, cost-effective, and chemically-defined tool for decontaminating hiPSC-derived neuron.

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SP - 1355

EP - 1358

JO - Chemical Communications

JF - Chemical Communications

IS - 11

ER -

Chemical decontamination of iPS cell-derived neural cell mixtures

Abstract

ASJC Scopus subject areas

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