Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells

Shigeyuki Magi, Yuya Saeki, Masato Kasamatsu, Etsu Tashiro, Masaya Imoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

To explore the diversity and consistency of the signaling pathways that regulate tumor cell migration, we chose three human tumor cell lines that migrated after treatment with EGF. We then quantified the effect of fifteen inhibitors on the levels of expression or the phosphorylation levels of nine proteins that were induced by EGF stimulation in each of these cell lines. Based on the data obtained in this study and chemical-biological assumptions, we deduced cell migration pathways in each tumor cell line, and then compared them. As a result, we found that both the MEK/ERK and JNK/c-Jun pathways were activated in all three migrating cell lines. Moreover, GSK-3 and p38 were found to regulate PI3K/Akt pathway in only EC109 cells, and JNK was found to crosstalk with p38 and Fos related pathway in only TT cells. Taken together, our analytical system could easily distinguish between the common and cell type-specific pathways responsible for tumor cell migration.

Original languageEnglish
Article numbere96776
JournalPLoS One
Volume9
Issue number5
DOIs
Publication statusPublished - 2014 May 12

Fingerprint

epidermal growth factor
Epidermal Growth Factor
Cell Movement
Cells
cell lines
cell movement
Tumor Cell Line
genomics
Tumors
Glycogen Synthase Kinase 3
Cell Line
Neoplasms
Mitogen-Activated Protein Kinase Kinases
Phosphatidylinositol 3-Kinases
phosphatidylinositol 3-kinase
cells
Phosphorylation
phosphorylation
Crosstalk
neoplasm cells

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chemical genomic-based pathway analyses for epidermal growth factor-mediated signaling in migrating cancer cells. / Magi, Shigeyuki; Saeki, Yuya; Kasamatsu, Masato; Tashiro, Etsu; Imoto, Masaya.

In: PLoS One, Vol. 9, No. 5, e96776, 12.05.2014.

Research output: Contribution to journalArticle

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