Chemo-enzymatic route for (R)-terbutaline hydrochloride based on microbial asymmetric reduction of a substituted α-chloroacetophenone derivative

Shohei Taketomi, Masayoshi Asano, Toshinori Higashi, Mitsuru Shoji, Takeshi Sugai

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To synthesize (R)-terbutaline hydrochloride, a potent β 2- adrenoceptor-stimulating agent, asymmetric reduction of a substituted α-chloroacetophenone derivative with cultured whole-cell biocatalyst of the yeast Williopsis californica JCM 3600 was developed as the key reaction. The reduction proceeded by a si-facial attack of hydride in a highly enantioselective manner. Co-factor generation was enhanced by applying glycerol as the carbon source.

Original languageEnglish
Pages (from-to)83-88
Number of pages6
JournalJournal of Molecular Catalysis B: Enzymatic
Volume84
DOIs
Publication statusPublished - 2012 Dec 1

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Keywords

  • Asymmetric reduction
  • Hydrolysis
  • Lipase
  • Yeast

ASJC Scopus subject areas

  • Catalysis
  • Bioengineering
  • Biochemistry
  • Process Chemistry and Technology

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