Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants

Junichi Enoki, Carolin Mügge, Dirk Tischler, Kenji Miyamoto, Robert Kourist

Research output: Contribution to journalArticle

Abstract

Arylmalonate decarboxylase (AMDase) catalyzes the cofactor-free asymmetric decarboxylation of prochiral arylmalonic acids and produces the corresponding monoacids with rigorous R selectivity. Alteration of catalytic cysteine residues and of the hydrophobic environment in the active site by protein engineering has previously resulted in the generation of variants with opposite enantioselectivity and improved catalytic performance. The substrate spectrum of AMDase allows it to catalyze the asymmetric decarboxylation of small methylvinylmalonic acid derivatives, implying the possibility to produce short-chain 2-methylalkanoic acids with high optical purity after reduction of the nonactivated C=C double bond. Use of diimide as the reductant proved to be a simple strategy to avoid racemization of the stereocenter during reduction. The developed chemoenzymatic sequential cascade with use of R- and S-selective AMDase variants produced optically pure short-chain 2-methylalkanoic acids in moderate to full conversion and gave both enantiomers in excellent enantiopurity (up to 83 % isolated yield and 98 % ee).

Original languageEnglish
Pages (from-to)5071-5076
Number of pages6
JournalChemistry - A European Journal
Volume25
Issue number19
DOIs
Publication statusPublished - 2019 Apr 1

Fingerprint

Carboxylic acids
Acids
Enantiomers
Enantioselectivity
Reducing Agents
Cysteine
Derivatives
Proteins
malonate decarboxylase
Substrates
Decarboxylation

Keywords

  • arylmalonate decarboxylase
  • biocatalysis
  • cascade reactions
  • enzymes
  • hydrogenation

ASJC Scopus subject areas

  • Catalysis
  • Organic Chemistry

Cite this

Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants. / Enoki, Junichi; Mügge, Carolin; Tischler, Dirk; Miyamoto, Kenji; Kourist, Robert.

In: Chemistry - A European Journal, Vol. 25, No. 19, 01.04.2019, p. 5071-5076.

Research output: Contribution to journalArticle

Enoki, Junichi ; Mügge, Carolin ; Tischler, Dirk ; Miyamoto, Kenji ; Kourist, Robert. / Chemoenzymatic Cascade Synthesis of Optically Pure Alkanoic Acids by Using Engineered Arylmalonate Decarboxylase Variants. In: Chemistry - A European Journal. 2019 ; Vol. 25, No. 19. pp. 5071-5076.
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