Chemokine-dependent T cell migration requires aquaporin-3-mediated hydrogen peroxide uptake

Mariko Chikuma, Shunsuke Chikuma, Yoshinori Sugiyama, Kenji Kabashima, Alan S. Verkman, Shintaro Inoue, Yoshiki Miyachi

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Chemokine-dependent trafficking is indispensable for the effector function of antigenexperienced T cells during immune responses. In this study, we report that the water/glycerol channel aquaporin-3 (AQP3) is expressed on T cells and regulates their trafficking in cutaneous immune reactions. T cell migration toward chemokines is dependent on AQP3-mediated hydrogen peroxide (H2O2) uptake but not the canonical water/glycerol transport. AQP3-mediated H2O2 transport is essential for the activation of the Rho family GTPase Cdc42 and the subsequent actin dynamics. Coincidentally, AQP3-deficient mice are defective in the development of hapten-induced contact hypersensitivity, which is attributed to the impaired trafficking of antigen-primed T cells to the hapten-challenged skin. We therefore suggest that AQP3-mediated H2O2 uptake is required for chemokine-dependent T cell migration in sufficient immune response.

Original languageEnglish
Pages (from-to)1743-1752
Number of pages10
JournalJournal of Experimental Medicine
Volume209
Issue number10
DOIs
Publication statusPublished - 2012 Sep
Externally publishedYes

Fingerprint

Aquaporin 3
Chemokines
Hydrogen Peroxide
Cell Movement
T-Lymphocytes
Haptens
Glycerol
rho GTP-Binding Proteins
Skin
Aquaporins
Contact Dermatitis
Actins
Antigens
Water

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Chemokine-dependent T cell migration requires aquaporin-3-mediated hydrogen peroxide uptake. / Chikuma, Mariko; Chikuma, Shunsuke; Sugiyama, Yoshinori; Kabashima, Kenji; Verkman, Alan S.; Inoue, Shintaro; Miyachi, Yoshiki.

In: Journal of Experimental Medicine, Vol. 209, No. 10, 09.2012, p. 1743-1752.

Research output: Contribution to journalArticle

Chikuma, Mariko ; Chikuma, Shunsuke ; Sugiyama, Yoshinori ; Kabashima, Kenji ; Verkman, Alan S. ; Inoue, Shintaro ; Miyachi, Yoshiki. / Chemokine-dependent T cell migration requires aquaporin-3-mediated hydrogen peroxide uptake. In: Journal of Experimental Medicine. 2012 ; Vol. 209, No. 10. pp. 1743-1752.
@article{0ff7d3d558b34f53b6913c2693e77589,
title = "Chemokine-dependent T cell migration requires aquaporin-3-mediated hydrogen peroxide uptake",
abstract = "Chemokine-dependent trafficking is indispensable for the effector function of antigenexperienced T cells during immune responses. In this study, we report that the water/glycerol channel aquaporin-3 (AQP3) is expressed on T cells and regulates their trafficking in cutaneous immune reactions. T cell migration toward chemokines is dependent on AQP3-mediated hydrogen peroxide (H2O2) uptake but not the canonical water/glycerol transport. AQP3-mediated H2O2 transport is essential for the activation of the Rho family GTPase Cdc42 and the subsequent actin dynamics. Coincidentally, AQP3-deficient mice are defective in the development of hapten-induced contact hypersensitivity, which is attributed to the impaired trafficking of antigen-primed T cells to the hapten-challenged skin. We therefore suggest that AQP3-mediated H2O2 uptake is required for chemokine-dependent T cell migration in sufficient immune response.",
author = "Mariko Chikuma and Shunsuke Chikuma and Yoshinori Sugiyama and Kenji Kabashima and Verkman, {Alan S.} and Shintaro Inoue and Yoshiki Miyachi",
year = "2012",
month = "9",
doi = "10.1084/jem.20112398",
language = "English",
volume = "209",
pages = "1743--1752",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "10",

}

TY - JOUR

T1 - Chemokine-dependent T cell migration requires aquaporin-3-mediated hydrogen peroxide uptake

AU - Chikuma, Mariko

AU - Chikuma, Shunsuke

AU - Sugiyama, Yoshinori

AU - Kabashima, Kenji

AU - Verkman, Alan S.

AU - Inoue, Shintaro

AU - Miyachi, Yoshiki

PY - 2012/9

Y1 - 2012/9

N2 - Chemokine-dependent trafficking is indispensable for the effector function of antigenexperienced T cells during immune responses. In this study, we report that the water/glycerol channel aquaporin-3 (AQP3) is expressed on T cells and regulates their trafficking in cutaneous immune reactions. T cell migration toward chemokines is dependent on AQP3-mediated hydrogen peroxide (H2O2) uptake but not the canonical water/glycerol transport. AQP3-mediated H2O2 transport is essential for the activation of the Rho family GTPase Cdc42 and the subsequent actin dynamics. Coincidentally, AQP3-deficient mice are defective in the development of hapten-induced contact hypersensitivity, which is attributed to the impaired trafficking of antigen-primed T cells to the hapten-challenged skin. We therefore suggest that AQP3-mediated H2O2 uptake is required for chemokine-dependent T cell migration in sufficient immune response.

AB - Chemokine-dependent trafficking is indispensable for the effector function of antigenexperienced T cells during immune responses. In this study, we report that the water/glycerol channel aquaporin-3 (AQP3) is expressed on T cells and regulates their trafficking in cutaneous immune reactions. T cell migration toward chemokines is dependent on AQP3-mediated hydrogen peroxide (H2O2) uptake but not the canonical water/glycerol transport. AQP3-mediated H2O2 transport is essential for the activation of the Rho family GTPase Cdc42 and the subsequent actin dynamics. Coincidentally, AQP3-deficient mice are defective in the development of hapten-induced contact hypersensitivity, which is attributed to the impaired trafficking of antigen-primed T cells to the hapten-challenged skin. We therefore suggest that AQP3-mediated H2O2 uptake is required for chemokine-dependent T cell migration in sufficient immune response.

UR - http://www.scopus.com/inward/record.url?scp=84870259348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84870259348&partnerID=8YFLogxK

U2 - 10.1084/jem.20112398

DO - 10.1084/jem.20112398

M3 - Article

C2 - 22927550

AN - SCOPUS:84870259348

VL - 209

SP - 1743

EP - 1752

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 10

ER -