Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point

T. Furukawa, T. Kubota, M. Watanabe, S. Kase, T. Takahara, H. Yamaguchi, T. Takeuchi, T. Teramoto, K. Ishibiki, M. Kitajima, R. M. Hoffman

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Abstract

Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.

Original languageEnglish
Pages (from-to)1377-1382
Number of pages6
JournalAnticancer research
Volume12
Issue number5
Publication statusPublished - 1992 Jan 1

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Furukawa, T., Kubota, T., Watanabe, M., Kase, S., Takahara, T., Yamaguchi, H., Takeuchi, T., Teramoto, T., Ishibiki, K., Kitajima, M., & Hoffman, R. M. (1992). Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point. Anticancer research, 12(5), 1377-1382.