Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point

Toshiharu Furukawa, T. Kubota, M. Watanabe, S. Kase, T. Takahara, H. Yamaguchi, T. Takeuchi, T. Teramoto, K. Ishibiki, M. Kitajima, R. M. Hoffman

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Abstract

Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.

Original languageEnglish
Pages (from-to)1377-1382
Number of pages6
JournalAnticancer Research
Volume12
Issue number5
Publication statusPublished - 1992

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Gastrointestinal Neoplasms
Suspensions
Cell Culture Techniques
Drug Therapy

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Furukawa, T., Kubota, T., Watanabe, M., Kase, S., Takahara, T., Yamaguchi, H., ... Hoffman, R. M. (1992). Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point. Anticancer Research, 12(5), 1377-1382.

Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point. / Furukawa, Toshiharu; Kubota, T.; Watanabe, M.; Kase, S.; Takahara, T.; Yamaguchi, H.; Takeuchi, T.; Teramoto, T.; Ishibiki, K.; Kitajima, M.; Hoffman, R. M.

In: Anticancer Research, Vol. 12, No. 5, 1992, p. 1377-1382.

Research output: Contribution to journalArticle

Furukawa, T, Kubota, T, Watanabe, M, Kase, S, Takahara, T, Yamaguchi, H, Takeuchi, T, Teramoto, T, Ishibiki, K, Kitajima, M & Hoffman, RM 1992, 'Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point', Anticancer Research, vol. 12, no. 5, pp. 1377-1382.
Furukawa T, Kubota T, Watanabe M, Kase S, Takahara T, Yamaguchi H et al. Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point. Anticancer Research. 1992;12(5):1377-1382.
Furukawa, Toshiharu ; Kubota, T. ; Watanabe, M. ; Kase, S. ; Takahara, T. ; Yamaguchi, H. ; Takeuchi, T. ; Teramoto, T. ; Ishibiki, K. ; Kitajima, M. ; Hoffman, R. M. / Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point. In: Anticancer Research. 1992 ; Vol. 12, No. 5. pp. 1377-1382.
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