Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point

T. Furukawa; T. Kubota; M. Watanabe; S. Kase; T. Takahara; H. Yamaguchi; T. Takeuchi; T. Teramoto; K. Ishibiki; M. Kitajima; R. M. Hoffman

Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point

T. Furukawa, T. Kubota, M. Watanabe, S. Kase, T. Takahara, H. Yamaguchi, T. Takeuchi, T. Teramoto, K. Ishibiki, M. Kitajima, R. M. Hoffman

Law School

Research output: Contribution to journal › Article › peer-review

31 Citations (Scopus)

Abstract

Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.

Original language	English
Pages (from-to)	1377-1382
Number of pages	6
Journal	Anticancer research
Volume	12
Issue number	5
Publication status	Published - 1992 Jan 1

ASJC Scopus subject areas

Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

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AU - Furukawa, T.

AU - Kubota, T.

AU - Watanabe, M.

AU - Kase, S.

AU - Takahara, T.

AU - Yamaguchi, H.

AU - Takeuchi, T.

AU - Teramoto, T.

AU - Ishibiki, K.

AU - Kitajima, M.

AU - Hoffman, R. M.

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N2 - Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.

AB - Three-dimensional histoculture with the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) end-point was utilized for chemosensitivity testing of 100 clinical gastrointestinal cancers and the results were compared with those obtained with cell-suspension culture with the MTT end-point. Of the 100 surgical specimens, 91 were evaluable using histoculture assay and 66 were evaluable using the cell-suspension assay. When the assay results were compared with historical frequency of clinical response to chemotherapy, the results of the histoculture assay showed a closer correlation than those of the cell-suspension assay. Therefore, the histoculture assay seems to have a higher evaluability rate and a closer correlation with clinical chemosensitivity of gastrointestinal cancers than the cell-suspension assay.

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Chemosensitivity testing of clinical gastrointestinal cancers using histoculture and the MTT end-point

Abstract

ASJC Scopus subject areas

UN SDGs

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