Chemotactic behavior of egg mitochondria in response to sperm fusion in mice

Maki Iwai, Yuichirou Harada, Rinako Miyabayashi, Woojin Kang, Akihiro Nakamura, Natsuko Kawano, Yoshitaka Miyamoto, Mitsutoshi Yamada, Toshio Hamatani, Mami Miyado, Keiichi Yoshida, Hidekazu Saito, Mamoru Tanaka, Akihiro Umezawa, Kenji Miyado

Research output: Contribution to journalArticle

Abstract

Mitochondria are the powerhouses of eukaryotic cells and their positioning contributes to fertilization and early developmental processes. We report that sperm fusion triggers Ca2+ oscillations and mitochondrial movement toward fused sperm (mitochondrial chemotaxis) in mouse eggs. Mitochondria functioned in Ca2+ storage and were colocalized with endoplasmic reticulum (ER) during Ca2+ oscillations. Mitochondria then moved toward the fused sperm. Sperm extracts lacking nuclei induced Ca2+ oscillations, but did not promote mitochondrial chemotaxis. Our results suggest that sperm fusion motivates Ca2+ oscillation-independent mitochondrial chemotaxis. This phenomenon indicates that egg mitochondria interact with sperm materials, presumably nuclear substances, and their network tethers egg and sperm nuclei at the early stage of zygote formation.

Original languageEnglish
Article numbere00944
JournalHeliyon
Volume4
Issue number11
DOIs
Publication statusPublished - 2018 Nov 1

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mitochondria
spermatozoa
mice
oscillation
chemotaxis
calcium
zygote
endoplasmic reticulum
eukaryotic cells
extracts

Keywords

  • Cell biology
  • Developmental biology
  • Molecular biology
  • Zoology

ASJC Scopus subject areas

  • General

Cite this

Iwai, M., Harada, Y., Miyabayashi, R., Kang, W., Nakamura, A., Kawano, N., ... Miyado, K. (2018). Chemotactic behavior of egg mitochondria in response to sperm fusion in mice. Heliyon, 4(11), [e00944]. https://doi.org/10.1016/j.heliyon.2018.e00944

Chemotactic behavior of egg mitochondria in response to sperm fusion in mice. / Iwai, Maki; Harada, Yuichirou; Miyabayashi, Rinako; Kang, Woojin; Nakamura, Akihiro; Kawano, Natsuko; Miyamoto, Yoshitaka; Yamada, Mitsutoshi; Hamatani, Toshio; Miyado, Mami; Yoshida, Keiichi; Saito, Hidekazu; Tanaka, Mamoru; Umezawa, Akihiro; Miyado, Kenji.

In: Heliyon, Vol. 4, No. 11, e00944, 01.11.2018.

Research output: Contribution to journalArticle

Iwai, M, Harada, Y, Miyabayashi, R, Kang, W, Nakamura, A, Kawano, N, Miyamoto, Y, Yamada, M, Hamatani, T, Miyado, M, Yoshida, K, Saito, H, Tanaka, M, Umezawa, A & Miyado, K 2018, 'Chemotactic behavior of egg mitochondria in response to sperm fusion in mice', Heliyon, vol. 4, no. 11, e00944. https://doi.org/10.1016/j.heliyon.2018.e00944
Iwai M, Harada Y, Miyabayashi R, Kang W, Nakamura A, Kawano N et al. Chemotactic behavior of egg mitochondria in response to sperm fusion in mice. Heliyon. 2018 Nov 1;4(11). e00944. https://doi.org/10.1016/j.heliyon.2018.e00944
Iwai, Maki ; Harada, Yuichirou ; Miyabayashi, Rinako ; Kang, Woojin ; Nakamura, Akihiro ; Kawano, Natsuko ; Miyamoto, Yoshitaka ; Yamada, Mitsutoshi ; Hamatani, Toshio ; Miyado, Mami ; Yoshida, Keiichi ; Saito, Hidekazu ; Tanaka, Mamoru ; Umezawa, Akihiro ; Miyado, Kenji. / Chemotactic behavior of egg mitochondria in response to sperm fusion in mice. In: Heliyon. 2018 ; Vol. 4, No. 11.
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