Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences

Wei Hao Shang, Tetsuya Hori, Atsushi Toyoda, Jun Kato, Kris Popendorf, Yasubumi Sakakibara, Asao Fujiyama, Tatsuo Fukagawa

Research output: Contribution to journalArticle

83 Citations (Scopus)

Abstract

The centromere is essential for faithful chromosome segregation by providing the site for kinetochore assembly. Although the role of the centromere is conserved throughout evolution, the DNA sequences associated with centromere regions are highly divergent among species and it remains to be determined how centromere DNA directs kinetochore formation. Despite the active use of chicken DT40 cells in studies of chromosome segregation, the sequence of the chicken centromere was unclear. Here, we performed a comprehensive analysis of chicken centromere DNA which revealed unique features of chicken centromeres compared with previously studied vertebrates. Centromere DNA sequences from the chicken macrochromosomes, with the exception of chromosome 5, contain chromosome-specific homogenous tandem repetitive arrays that span several hundred kilobases. In contrast, the centromeres of chromosomes 5, 27, and Z do not contain tandem repetitive sequences and span non-tandem-repetitive sequences of only ∼30 kb. To test the function of these centromere sequences, we conditionally removed the centromere from the Z chromosome using genetic engineering and have shown that that the non-tandem-repeat sequence of chromosome Z is a functional centromere.

Original languageEnglish
Pages (from-to)1219-1228
Number of pages10
JournalGenome Research
Volume20
Issue number9
DOIs
Publication statusPublished - 2010 Sep

Fingerprint

Centromere
Nucleic Acid Repetitive Sequences
Microsatellite Repeats
Chickens
Kinetochores
Chromosome Segregation
Chromosomes, Human, Pair 5
Chromosomes
Genetic Engineering
DNA
Vertebrates

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

Cite this

Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences. / Shang, Wei Hao; Hori, Tetsuya; Toyoda, Atsushi; Kato, Jun; Popendorf, Kris; Sakakibara, Yasubumi; Fujiyama, Asao; Fukagawa, Tatsuo.

In: Genome Research, Vol. 20, No. 9, 09.2010, p. 1219-1228.

Research output: Contribution to journalArticle

Shang, WH, Hori, T, Toyoda, A, Kato, J, Popendorf, K, Sakakibara, Y, Fujiyama, A & Fukagawa, T 2010, 'Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences', Genome Research, vol. 20, no. 9, pp. 1219-1228. https://doi.org/10.1101/gr.106245.110
Shang, Wei Hao ; Hori, Tetsuya ; Toyoda, Atsushi ; Kato, Jun ; Popendorf, Kris ; Sakakibara, Yasubumi ; Fujiyama, Asao ; Fukagawa, Tatsuo. / Chickens possess centromeres with both extended tandem repeats and short non-tandem-repetitive sequences. In: Genome Research. 2010 ; Vol. 20, No. 9. pp. 1219-1228.
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