Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos

Michael Oelgeschläger, Hiroki Kuroda, Bruno Reversade, E. M. De Robertis

Research output: Contribution to journalArticle

113 Citations (Scopus)

Abstract

We analyzed the Chordin requirement in Xenopus development. Targeting of both chordin Xenopus laevis pseudoalleles with morpholino antisense oligomers (Chd-MO) markedly decreased Chordin production. Embryos developed with moderately reduced dorsoanterior structures and expanded ventroposterior tissues, phenocopying the zebrafish chordino mutant. A strong requirement for Chordin in dorsal development was revealed by experimental manipulations. First, dorsalization by lithium chloride treatment was completely blocked by Chd-MO. Second, Chd-MO inhibited elongation and muscle differentiation in Activin-treated animal caps. Third, Chd-MO completely blocked the induction of the central nervous system (CNS), somites, and notochord by organizer tissue transplanted to the ventral side of host embryos. Unexpectedly, transplantations into the dorsal side revealed a cell-autonomous requirement of Chordin for neural plate differentiation.

Original languageEnglish
Pages (from-to)219-230
Number of pages12
JournalDevelopmental Cell
Volume4
Issue number2
DOIs
Publication statusPublished - 2003 Feb 1
Externally publishedYes

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Embryonic Organizers
Xenopus
Embryonic Structures
Transplantation
Tissue
Neural Plate
Lithium Chloride
Notochord
Activins
Morpholinos
Somites
Xenopus laevis
Neurology
Zebrafish
Oligomers
Muscle
Elongation
Animals
Central Nervous System
chordin

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos. / Oelgeschläger, Michael; Kuroda, Hiroki; Reversade, Bruno; De Robertis, E. M.

In: Developmental Cell, Vol. 4, No. 2, 01.02.2003, p. 219-230.

Research output: Contribution to journalArticle

Oelgeschläger, Michael ; Kuroda, Hiroki ; Reversade, Bruno ; De Robertis, E. M. / Chordin is required for the spemann organizer transplantation phenomenon in Xenopus embryos. In: Developmental Cell. 2003 ; Vol. 4, No. 2. pp. 219-230.
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