Chromatid break rejoining and exchange aberration formation following γ-ray exposure

Analysis in G2 human fibroblasts by chemically induced premature chromosome condensation

Eisuke Gotoh, Tetsuya Kawata, Marco Durante

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Purpose: To analyse the kinetics of chromatid break induction, rejoining, and misrejoining after γ-irradiation in G2 phase human cells using premature chromosome condensation induced by calyculin A. Materials and methods: Human fibroblast AG1522 cells were irradiated with γ-rays and chromosomes were then prematurely condensed by calyculin A. The number of chromatid breaks and chromatid exchanges in G2 chromosomes were scored, and fitted curves were calculated. Results: Calyculin A induced premature chromosome condensation in cells immediately after irradiation. Kinetics of rejoining of chromatid breaks demonstrated two exponential components with rapid and slow time constants. Within 5 min after irradiation, the number of chromatid breaks fell rapidly to about one-half, then gradually decreased. Chromatid exchanges were formed very quickly, reaching a plateau within 20 min from exposure. Conclusions: Chemically induced premature chromosome condensation technique allows a simple, rapid and precise analysis of chromatid breakage and rejoining. The rapid kinetic component was particularly well characterized.

Original languageEnglish
Pages (from-to)1129-1135
Number of pages7
JournalInternational Journal of Radiation Biology
Volume75
Issue number9
DOIs
Publication statusPublished - 1999

Fingerprint

exposure assessment
Chromatids
chromatids
chromosomes
fibroblasts
Fibroblasts
Chromosomes
Aberrations
aberration
Condensation
rays
condensation
Irradiation
Kinetics
irradiation
kinetics
cells
time constant
plateaus
induction

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology
  • Nuclear Energy and Engineering
  • Radiation

Cite this

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title = "Chromatid break rejoining and exchange aberration formation following γ-ray exposure: Analysis in G2 human fibroblasts by chemically induced premature chromosome condensation",
abstract = "Purpose: To analyse the kinetics of chromatid break induction, rejoining, and misrejoining after γ-irradiation in G2 phase human cells using premature chromosome condensation induced by calyculin A. Materials and methods: Human fibroblast AG1522 cells were irradiated with γ-rays and chromosomes were then prematurely condensed by calyculin A. The number of chromatid breaks and chromatid exchanges in G2 chromosomes were scored, and fitted curves were calculated. Results: Calyculin A induced premature chromosome condensation in cells immediately after irradiation. Kinetics of rejoining of chromatid breaks demonstrated two exponential components with rapid and slow time constants. Within 5 min after irradiation, the number of chromatid breaks fell rapidly to about one-half, then gradually decreased. Chromatid exchanges were formed very quickly, reaching a plateau within 20 min from exposure. Conclusions: Chemically induced premature chromosome condensation technique allows a simple, rapid and precise analysis of chromatid breakage and rejoining. The rapid kinetic component was particularly well characterized.",
author = "Eisuke Gotoh and Tetsuya Kawata and Marco Durante",
year = "1999",
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TY - JOUR

T1 - Chromatid break rejoining and exchange aberration formation following γ-ray exposure

T2 - Analysis in G2 human fibroblasts by chemically induced premature chromosome condensation

AU - Gotoh, Eisuke

AU - Kawata, Tetsuya

AU - Durante, Marco

PY - 1999

Y1 - 1999

N2 - Purpose: To analyse the kinetics of chromatid break induction, rejoining, and misrejoining after γ-irradiation in G2 phase human cells using premature chromosome condensation induced by calyculin A. Materials and methods: Human fibroblast AG1522 cells were irradiated with γ-rays and chromosomes were then prematurely condensed by calyculin A. The number of chromatid breaks and chromatid exchanges in G2 chromosomes were scored, and fitted curves were calculated. Results: Calyculin A induced premature chromosome condensation in cells immediately after irradiation. Kinetics of rejoining of chromatid breaks demonstrated two exponential components with rapid and slow time constants. Within 5 min after irradiation, the number of chromatid breaks fell rapidly to about one-half, then gradually decreased. Chromatid exchanges were formed very quickly, reaching a plateau within 20 min from exposure. Conclusions: Chemically induced premature chromosome condensation technique allows a simple, rapid and precise analysis of chromatid breakage and rejoining. The rapid kinetic component was particularly well characterized.

AB - Purpose: To analyse the kinetics of chromatid break induction, rejoining, and misrejoining after γ-irradiation in G2 phase human cells using premature chromosome condensation induced by calyculin A. Materials and methods: Human fibroblast AG1522 cells were irradiated with γ-rays and chromosomes were then prematurely condensed by calyculin A. The number of chromatid breaks and chromatid exchanges in G2 chromosomes were scored, and fitted curves were calculated. Results: Calyculin A induced premature chromosome condensation in cells immediately after irradiation. Kinetics of rejoining of chromatid breaks demonstrated two exponential components with rapid and slow time constants. Within 5 min after irradiation, the number of chromatid breaks fell rapidly to about one-half, then gradually decreased. Chromatid exchanges were formed very quickly, reaching a plateau within 20 min from exposure. Conclusions: Chemically induced premature chromosome condensation technique allows a simple, rapid and precise analysis of chromatid breakage and rejoining. The rapid kinetic component was particularly well characterized.

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