TY - JOUR
T1 - Chromatid break rejoining and exchange aberration formation following γ-ray exposure
T2 - Analysis in G2 human fibroblasts by chemically induced premature chromosome condensation
AU - Gotoh, Eisuke
AU - Kawata, Tetsuya
AU - Durante, Marco
N1 - Funding Information:
T. K is supported by National Research Council (USA) Fellowship No. 918. 7M1. D08. was supported by the Sciences and Technology Agency ( Japan) Fellowship No. 129. 161
PY - 1999
Y1 - 1999
N2 - Purpose: To analyse the kinetics of chromatid break induction, rejoining, and misrejoining after γ-irradiation in G2 phase human cells using premature chromosome condensation induced by calyculin A. Materials and methods: Human fibroblast AG1522 cells were irradiated with γ-rays and chromosomes were then prematurely condensed by calyculin A. The number of chromatid breaks and chromatid exchanges in G2 chromosomes were scored, and fitted curves were calculated. Results: Calyculin A induced premature chromosome condensation in cells immediately after irradiation. Kinetics of rejoining of chromatid breaks demonstrated two exponential components with rapid and slow time constants. Within 5 min after irradiation, the number of chromatid breaks fell rapidly to about one-half, then gradually decreased. Chromatid exchanges were formed very quickly, reaching a plateau within 20 min from exposure. Conclusions: Chemically induced premature chromosome condensation technique allows a simple, rapid and precise analysis of chromatid breakage and rejoining. The rapid kinetic component was particularly well characterized.
AB - Purpose: To analyse the kinetics of chromatid break induction, rejoining, and misrejoining after γ-irradiation in G2 phase human cells using premature chromosome condensation induced by calyculin A. Materials and methods: Human fibroblast AG1522 cells were irradiated with γ-rays and chromosomes were then prematurely condensed by calyculin A. The number of chromatid breaks and chromatid exchanges in G2 chromosomes were scored, and fitted curves were calculated. Results: Calyculin A induced premature chromosome condensation in cells immediately after irradiation. Kinetics of rejoining of chromatid breaks demonstrated two exponential components with rapid and slow time constants. Within 5 min after irradiation, the number of chromatid breaks fell rapidly to about one-half, then gradually decreased. Chromatid exchanges were formed very quickly, reaching a plateau within 20 min from exposure. Conclusions: Chemically induced premature chromosome condensation technique allows a simple, rapid and precise analysis of chromatid breakage and rejoining. The rapid kinetic component was particularly well characterized.
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U2 - 10.1080/095530099139601
DO - 10.1080/095530099139601
M3 - Article
C2 - 10528921
AN - SCOPUS:0032860448
SN - 0955-3002
VL - 75
SP - 1129
EP - 1135
JO - International Journal of Radiation Biology
JF - International Journal of Radiation Biology
IS - 9
ER -