Chromatographic separation and identification of human urine components

Y. Nagata, Y. Matsushima, H. Ugai, M. Kikuchi, N. Takai

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Abstract

Ultraviolet-absorbing components of human urine were separated by HPLC with a large scale column (250 × 28 mm I.D. stainless-steel) containing a macroreticular type strongly basic anion-exchange resin. The sample was eluted with a linear gradient of 0.006 M to 6.0 M ammonium acetate buffer (pH 4.40) and different fractions were collected. The identification of one compound using ultraviolet, infrared and mass spectral data is illustrated; it was found to be uric acid.

Original languageEnglish
Pages (from-to)317-318
Number of pages2
JournalChromatographia
Volume24
Issue number1
DOIs
Publication statusPublished - 1987 Dec 1

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Keywords

  • Human urine
  • Large scale column liquid chromatography
  • Strongly basic anion-exchange resin
  • Uric acid

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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