Chromosomal mapping of a family of human glutamine synthetase genes: Functional gene (GLUL) on 1q25, pseudogene (GLULP) on 9p13, and three related genes (GLULL1, GLULL2, GLULL3) on 5q33, 11p15, and 11q24

Yimin Wang, Jun Kudo, Ryo Kubota, Shuichi Asakawa, Shinsei Minoshima, Nobuyoshi Shimizu

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Abstract

Glutamine synthetase (GS) is a ubiquitous enzyme that catalyzes the ATP- dependent conversion of glutamate to glutamine using ammonia as the nitrogen source. Using human GS cDNA as a probe, a bacterial artificial chromosome (BAC) library consisting of twofold coverage of the human genome was screened, and 18 clones were obtained. The restriction analysis of the human insert DNAs provided a basis to divide these 18 BAC clones into five groups, suggesting the existence of a GS gene family in the human genome. PCR analysis using three sets of primers designed from the reported sequences for GS cDNA and a processed pseudogene identified the corresponding BAC clones. Fluorescence in situ hybridization analysis revealed the chromosomal localization of these five genes: the GS gene to 1q25 (GLUL), the processed pseudogene to 9p13 (GLULP), and three related genes to 5q33 (GLULL1), 11p15 (GLULL2), and 11q24 (GLULL3), respectively.

Original languageEnglish
Pages (from-to)195-199
Number of pages5
JournalGenomics
Volume37
Issue number2
DOIs
Publication statusPublished - 1996 Oct 15

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ASJC Scopus subject areas

  • Genetics

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