Chromosome spread analyses of meiotic sex chromosome inactivation

Kris G. Alavattam, Hironori Abe, Akihiko Sakashita, Satoshi H. Namekawa

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Citations (Scopus)

Abstract

A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.

Original languageEnglish
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages113-129
Number of pages17
DOIs
Publication statusPublished - 2018
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume1861
ISSN (Print)1064-3745

Keywords

  • Cell staging
  • Cell suspension
  • Chromatin
  • Chromosomes
  • Cytology
  • DNA damage response
  • Immunofluorescence microscopy
  • Meiosis
  • Sex chromosomes
  • Slide protocol
  • Synapsis
  • Testis

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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