@inbook{dfeac091cda6468082e7291881b72e2e,
title = "Chromosome spread analyses of meiotic sex chromosome inactivation",
abstract = "A distinct form of X chromosome inactivation takes place during male meiosis, when the male sex chromosomes undergo a phenomenon known as meiotic sex chromosome inactivation (MSCI). MSCI is directed by DNA damage response signaling independent of Xist RNA to silence the transcriptional activity of the sex chromosomes, an essential event in male germ cell development. Here, we present protocols for the preparation and analyses of chromosome spread slides of mouse meiotic spermatocytes, thereby enabling a quick, inexpensive, and powerful cytological method to complement gene expression studies.",
keywords = "Cell staging, Cell suspension, Chromatin, Chromosomes, Cytology, DNA damage response, Immunofluorescence microscopy, Meiosis, Sex chromosomes, Slide protocol, Synapsis, Testis",
author = "Alavattam, {Kris G.} and Hironori Abe and Akihiko Sakashita and Namekawa, {Satoshi H.}",
note = "Funding Information: We thank past and present members of the Namekawa laboratory for optimizing the experimental conditions. We thank Anna Sosso for reviewing the chapter. This work was supported by the Albert J. Ryan Fellowship to K.G.A. and the NIH Grant GM098605 to S.H.N. Publisher Copyright: {\textcopyright} Springer Science+Business Media, LLC, part of Springer Nature 2018.",
year = "2018",
doi = "10.1007/978-1-4939-8766-5_10",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "113--129",
booktitle = "Methods in Molecular Biology",
}