Chronic ethanol consumption enhances endotoxin induced hepatic sinusoidal leukocyte adhesion

E. Ohki, Shinzo Kato, Y. Horie, T. Mizukami, H. Tamai, Hirokazu Yokoyama, D. Ito, M. Fukuda, Hidekazu Suzuki, I. Kurose, H. Ishii

Research output: Contribution to journalArticle

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Abstract

In alcoholic liver disease, endotoxin has been postulated to play an important role in its pathogenesis. Endotoxin is known to lead to impediment of hepatic microcirculation, including the adhesion of leukocytes to sinusoidal endothelial cells. In this study, the effect of chronic ethanol consumption on the leukocyte adhesion elicited by endotoxin was examined. Male Wistar rats were pair-fed with s liquid diet containing ethanol or an isocaloric control diet for 6 weeks. The liver of anesthetized rats were placed on the nonfluorescent coverglass for observation by an intravital inverted microscope equipped with a silicon intensified target camera. The red blood cell (RBC) velocity in hepatic sinusoids was measured by an off- line temporal correlation velocimeter (Capiflow, Sweden) after intravenous injection of fluorescein isothiocyanate-labeled rat RBC. RBC velocity in sinusoids was more severely disturbed in ethanol fed rats than in controls. Leukocytes were stained by the intravenous injection of carboxyfluorescein succinimidyl ester for a fluorographic observation of leukocyte adhesion. After lipopolysaccharide injection, the number of adherent leukocytes was significantly greater in ethanol-fed rats than in controls. Plasma tumor necrosis factor-α levels were also higher in ethanol-fed rats than in controls. These results suggest that chronic ethanol consumption aggravates ondotoxin induced leukocytes adhesion that may result in hepatic microcirculatory disturbances. Leukocyte adhesion to the sinusoidal wall may be associated with increased in tumor necrosis factor-α levels.

Original languageEnglish
JournalAlcoholism: Clinical and Experimental Research
Volume20
Issue number9 SUPPL.
DOIs
Publication statusPublished - 1996

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Endotoxins
Rats
Leukocytes
Ethanol
Adhesion
Liver
Blood
Erythrocytes
Nutrition
Intravenous Injections
Tumor Necrosis Factor-alpha
Observation
Diet
Microcirculation
Velocimeters
Alcoholic Liver Diseases
Endothelial cells
Silicon
Fluorescein
Leukocyte Count

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Toxicology

Cite this

Chronic ethanol consumption enhances endotoxin induced hepatic sinusoidal leukocyte adhesion. / Ohki, E.; Kato, Shinzo; Horie, Y.; Mizukami, T.; Tamai, H.; Yokoyama, Hirokazu; Ito, D.; Fukuda, M.; Suzuki, Hidekazu; Kurose, I.; Ishii, H.

In: Alcoholism: Clinical and Experimental Research, Vol. 20, No. 9 SUPPL., 1996.

Research output: Contribution to journalArticle

Ohki, E. ; Kato, Shinzo ; Horie, Y. ; Mizukami, T. ; Tamai, H. ; Yokoyama, Hirokazu ; Ito, D. ; Fukuda, M. ; Suzuki, Hidekazu ; Kurose, I. ; Ishii, H. / Chronic ethanol consumption enhances endotoxin induced hepatic sinusoidal leukocyte adhesion. In: Alcoholism: Clinical and Experimental Research. 1996 ; Vol. 20, No. 9 SUPPL.
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