CINC/gro and MCP-1 induce CD18-dependent neutrophil migration from inflamed venules in vivo

B. Johnston, A. R. Burns, M. Suematsu, T. B. Issekutz, P. Kubes

Research output: Contribution to journalArticle

Abstract

In a model of adjuvant-induced vasculitis, we have reported a large increase in leukocyte trafficking within mesenteric postcapillary venules (J.Exp.Med. 183:1995). However, leukocyte emigration from these vessels was very low. In this study we examined the adhesion molecules that mediate transendothelial leukocyte migration in response to the C-X-C chemokine CINC/gro and the C-C chemokine MCP-1. CINC/gro (1 nM) and MCP-1 (1 ng/ml) did not elicit leukocyte diapedesis in the mesentery of control rats, but caused large increases in leukocyte diapedesis in adjuvant-immunized rats (MCP-1: 61.8±9.8, CINC/gro:101.3±8.5 cells/field). Histology revealed that the majority of the recruited leukocytes were neutrophils. Moreover, an anti-neutrophil serum significantly reduced both MCP-1 (18.3±4.8 cells/field) and CINC/gro-induced (29.7±8.2 cells/field) leukocyte emigration. An anti-CD18 antibody almost completely blocked MCP-1-induced leukocyte emigration (9.0±1.0 cells/field), but did not affect leukocyte adhesion. An antibody against the ∝4-integrin (CD49d) reduced both adhesion and emigration (22.0±17.5 cells/field). This study demonstrates that both C-X-C and C-C chemokines can recruit neutrophils in vivo, and that CD18 is required for transendothelial migration independent from its usual role in leukocyte adhesion.

Original languageEnglish
JournalFASEB Journal
Volume12
Issue number5
Publication statusPublished - 1998 Mar 20

Fingerprint

Venules
1-methylcyclopropene
neutrophils
leukocytes
Neutrophils
Leukocytes
Adhesion
CC Chemokines
Transendothelial and Transepithelial Migration
Emigration and Immigration
Rat control
adhesion
CXC Chemokines
Histology
Antibodies
Integrins
Rats
adjuvants
cells
Molecules

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Johnston, B., Burns, A. R., Suematsu, M., Issekutz, T. B., & Kubes, P. (1998). CINC/gro and MCP-1 induce CD18-dependent neutrophil migration from inflamed venules in vivo. FASEB Journal, 12(5).

CINC/gro and MCP-1 induce CD18-dependent neutrophil migration from inflamed venules in vivo. / Johnston, B.; Burns, A. R.; Suematsu, M.; Issekutz, T. B.; Kubes, P.

In: FASEB Journal, Vol. 12, No. 5, 20.03.1998.

Research output: Contribution to journalArticle

Johnston, B, Burns, AR, Suematsu, M, Issekutz, TB & Kubes, P 1998, 'CINC/gro and MCP-1 induce CD18-dependent neutrophil migration from inflamed venules in vivo', FASEB Journal, vol. 12, no. 5.
Johnston B, Burns AR, Suematsu M, Issekutz TB, Kubes P. CINC/gro and MCP-1 induce CD18-dependent neutrophil migration from inflamed venules in vivo. FASEB Journal. 1998 Mar 20;12(5).
Johnston, B. ; Burns, A. R. ; Suematsu, M. ; Issekutz, T. B. ; Kubes, P. / CINC/gro and MCP-1 induce CD18-dependent neutrophil migration from inflamed venules in vivo. In: FASEB Journal. 1998 ; Vol. 12, No. 5.
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