CISH controls bacterial burden early after infection with Mycobacterium tuberculosis in mice

Berit Carow; Yu Gao; Graciela Terán; Xuexian O. Yang; Chen Dong; Akihiko Yoshimura; Martin E. Rottenberg

doi:10.1016/j.tube.2017.09.007

CISH controls bacterial burden early after infection with Mycobacterium tuberculosis in mice

Berit Carow, Yu Gao, Graciela Terán, Xuexian O. Yang, Chen Dong, Akihiko Yoshimura, Martin E. Rottenberg

Department of Microbiology and Immunology

Research output: Contribution to journal › Article › peer-review

8 Citations (Scopus)

Abstract

CISH gene has been associated with increased susceptibility to human tuberculosis. We found that cish^−/− mice had higher M. tuberculosis load in spleens and lungs up to 2.5 weeks after infection but not later compared to controls. Cish mRNA levels were increased in lungs at early and late time points after M. tuberculosis infection. In relation, the titers of inos and tnf mRNA in lungs were reduced early after infection of cish^−/− mice. The transfer of cish^−/− and control T cells conferred rag1^−/− mice similar protection to infection with M. tuberculosis. Macrophages showed increased cish mRNA levels after M. tuberculosis infection in vitro. However, mycobacterial uptake and growth in cish^−/− and control macrophages was similar. Thus, we here show that CISH mediates control of M. tuberculosis in mice early after infection via regulation of innate immune mechanisms.

Original language	English
Pages (from-to)	175-180
Number of pages	6
Journal	Tuberculosis
Volume	107
DOIs	https://doi.org/10.1016/j.tube.2017.09.007
Publication status	Published - 2017 Dec

Keywords

Cytokine-inducible SRC homology 2 domain gene
Cytokines
Macrophage
Mycobacterium tuberculosis
SOCS
T cell

ASJC Scopus subject areas

Microbiology
Immunology
Microbiology (medical)
Infectious Diseases

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.tube.2017.09.007

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title = "CISH controls bacterial burden early after infection with Mycobacterium tuberculosis in mice",

abstract = "CISH gene has been associated with increased susceptibility to human tuberculosis. We found that cish−/− mice had higher M. tuberculosis load in spleens and lungs up to 2.5 weeks after infection but not later compared to controls. Cish mRNA levels were increased in lungs at early and late time points after M. tuberculosis infection. In relation, the titers of inos and tnf mRNA in lungs were reduced early after infection of cish−/− mice. The transfer of cish−/− and control T cells conferred rag1−/− mice similar protection to infection with M. tuberculosis. Macrophages showed increased cish mRNA levels after M. tuberculosis infection in vitro. However, mycobacterial uptake and growth in cish−/− and control macrophages was similar. Thus, we here show that CISH mediates control of M. tuberculosis in mice early after infection via regulation of innate immune mechanisms.",

keywords = "Cytokine-inducible SRC homology 2 domain gene, Cytokines, Macrophage, Mycobacterium tuberculosis, SOCS, T cell",

author = "Berit Carow and Yu Gao and Graciela Ter{\'a}n and Yang, {Xuexian O.} and Chen Dong and Akihiko Yoshimura and Rottenberg, {Martin E.}",

note = "Funding Information: This study was supported by the Swedish Heart and Lung Foundation 2015-17/20140641 , the Swedish Research Council 2015-02296 and the Swedish Institute for Internationalization of Research (STINT) 4-1796/2014 to MER, and the Karolinska Institutet (MER and BC). Publisher Copyright: {\textcopyright} 2017 Elsevier Ltd",

year = "2017",

month = dec,

doi = "10.1016/j.tube.2017.09.007",

language = "English",

volume = "107",

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AU - Carow, Berit

AU - Gao, Yu

AU - Terán, Graciela

AU - Yang, Xuexian O.

AU - Dong, Chen

AU - Yoshimura, Akihiko

AU - Rottenberg, Martin E.

N1 - Funding Information: This study was supported by the Swedish Heart and Lung Foundation 2015-17/20140641 , the Swedish Research Council 2015-02296 and the Swedish Institute for Internationalization of Research (STINT) 4-1796/2014 to MER, and the Karolinska Institutet (MER and BC). Publisher Copyright: © 2017 Elsevier Ltd

PY - 2017/12

Y1 - 2017/12

N2 - CISH gene has been associated with increased susceptibility to human tuberculosis. We found that cish−/− mice had higher M. tuberculosis load in spleens and lungs up to 2.5 weeks after infection but not later compared to controls. Cish mRNA levels were increased in lungs at early and late time points after M. tuberculosis infection. In relation, the titers of inos and tnf mRNA in lungs were reduced early after infection of cish−/− mice. The transfer of cish−/− and control T cells conferred rag1−/− mice similar protection to infection with M. tuberculosis. Macrophages showed increased cish mRNA levels after M. tuberculosis infection in vitro. However, mycobacterial uptake and growth in cish−/− and control macrophages was similar. Thus, we here show that CISH mediates control of M. tuberculosis in mice early after infection via regulation of innate immune mechanisms.

AB - CISH gene has been associated with increased susceptibility to human tuberculosis. We found that cish−/− mice had higher M. tuberculosis load in spleens and lungs up to 2.5 weeks after infection but not later compared to controls. Cish mRNA levels were increased in lungs at early and late time points after M. tuberculosis infection. In relation, the titers of inos and tnf mRNA in lungs were reduced early after infection of cish−/− mice. The transfer of cish−/− and control T cells conferred rag1−/− mice similar protection to infection with M. tuberculosis. Macrophages showed increased cish mRNA levels after M. tuberculosis infection in vitro. However, mycobacterial uptake and growth in cish−/− and control macrophages was similar. Thus, we here show that CISH mediates control of M. tuberculosis in mice early after infection via regulation of innate immune mechanisms.

KW - Cytokine-inducible SRC homology 2 domain gene

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KW - Macrophage

KW - Mycobacterium tuberculosis

KW - SOCS

KW - T cell

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ER -

CISH controls bacterial burden early after infection with Mycobacterium tuberculosis in mice

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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