Cis/trans-activation of the interleukin-9 receptor gene in an HTLV-I-transformed human lymphocytic cell

Satoshi Kubota, Haruhiko Siomi, Masakazu Hatanaka, Roger J. Pomerantz

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The MT-2 cell-line, which had been established through in vitro cell to cell transmission of human T-cell leukemia virus type I (HTLV-I) among human primary lymphocytes, was shown to possess multiple copies of integrated proviruses, including defective proviral genomes. By analysing a genomic clone, we identified the integration site of a single HTLV-I long terminal repeat (LTR) in the interleukin-9 (IL-9) receptor (IL-9R) gene. The integrated HTLV-I-LTR was shown to be functional as a promoter and the integration site was located in an intron upstream of the first coding exon of the IL-9R gene. Upon analysis of total cellular RNA, specific expression of HTLV-I-LTR IL-9R chimeric mRNAs in MT-2 cells was demonstrated. Cloning and characterization of these cDNAs have identified HTLV-I-IL-9R chimeric splicing, using either intact or alternative splice sites within the IL-9R gene. The potential roles of multiple interactions between LL-9, IL-9R and HTLV-I in the monoclonal expansion and transformation of MT-2 cells are explored.

Original languageEnglish
Pages (from-to)1441-1447
Number of pages7
JournalOncogene
Volume12
Issue number7
Publication statusPublished - 1996

Keywords

  • HIV-1
  • HTLV-I
  • Interleukin-9
  • Long terminal repeat
  • Receptor
  • T-lymphocyte

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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