Class-specific binding of two aminoacyl-tRNA synthetases to annexin, a Ca2+-and phospholipid-binding protein

Tomohiro Shirakawa, Akio Nakamura, Kazuhiro Kohama, Michito Hirakata, Satoshi Ogihara

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Annexins are a family of Ca2+/phospholipid-binding proteins that have diverse functions. To understand the function of annexin in Physarum polycephalum, we searched for its binding proteins. Here we demonstrate the presence of two novel annexin-binding proteins. The homology search of partial amino acid sequences of these two proteins identified them as aminoacyl-tRNA synthetases (ARSs). Furthermore, antibody against aminoacyl-tRNA synthetases cross-reacted with one of two proteins. Our results imply the interaction between intracellular membrane dynamics and protein translation system, and may give a clue to understand the mechanism of some myositis diseases, which have been known to produce autoantibodies against ARSs.

Original languageEnglish
Pages (from-to)159-164
Number of pages6
JournalCell Structure and Function
Volume29
Issue number5-6
DOIs
Publication statusPublished - 2005 Feb

Fingerprint

Annexins
Amino Acyl-tRNA Synthetases
Phospholipids
Carrier Proteins
Physarum polycephalum
Intracellular Membranes
Myositis
Protein Biosynthesis
Autoantibodies
Amino Acid Sequence
Membrane Proteins
Proteins
Antibodies

Keywords

  • Aminoacyl-tRNA synthetase
  • Annexin
  • Myositis
  • Physarum polycephalum

ASJC Scopus subject areas

  • Physiology
  • Structural Biology
  • Cell Biology

Cite this

Class-specific binding of two aminoacyl-tRNA synthetases to annexin, a Ca2+-and phospholipid-binding protein. / Shirakawa, Tomohiro; Nakamura, Akio; Kohama, Kazuhiro; Hirakata, Michito; Ogihara, Satoshi.

In: Cell Structure and Function, Vol. 29, No. 5-6, 02.2005, p. 159-164.

Research output: Contribution to journalArticle

Shirakawa, Tomohiro ; Nakamura, Akio ; Kohama, Kazuhiro ; Hirakata, Michito ; Ogihara, Satoshi. / Class-specific binding of two aminoacyl-tRNA synthetases to annexin, a Ca2+-and phospholipid-binding protein. In: Cell Structure and Function. 2005 ; Vol. 29, No. 5-6. pp. 159-164.
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