Cleavable linker for photo-cross-linked small-molecule affinity matrix

Naoki Kanoh, Hiroshi Takayama, Kaori Honda, Takashi Moriya, Takayuki Teruya, Siro Simizu, Hiroyuki Osada, Yoshiharu Iwabuchi

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The introduction of a cleavable site in a photoactivatable linker, which is used to immobilize small molecules on an affinity matrix via a site-nonselective carbene addition/insertion reaction, makes it possible to verify the presence of the immobilized small molecule on the affinity matrix. It also permits the efficient detection of proteins covalently bound to the immobilized small molecule.

Original languageEnglish
Pages (from-to)182-186
Number of pages5
JournalBioconjugate Chemistry
Volume21
Issue number1
DOIs
Publication statusPublished - 2010 Jan 20
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Pharmacology
  • Pharmaceutical Science
  • Organic Chemistry

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  • Cite this

    Kanoh, N., Takayama, H., Honda, K., Moriya, T., Teruya, T., Simizu, S., Osada, H., & Iwabuchi, Y. (2010). Cleavable linker for photo-cross-linked small-molecule affinity matrix. Bioconjugate Chemistry, 21(1), 182-186. https://doi.org/10.1021/bc900316q