Cleavage from the N-terminal region of βBp crystallin during aging of the human lens

L. Takemoto, D. Takemoto, G. Brown, M. Takehana, J. Smith, J. Horwitz

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Polyclonal antisera have been made to synthetic peptides that correspond to the N-terminal (residues 1-12) and C-terminal (residues 195-204) sequences of bovine βBp crystallin. Both antiβBp1-12 and anti-βBp195-204 recognize specifically the βBp component of bovine lens. In the young human lens, anti-βBp195-204 recognizes predominantly the 26000 MW form of βBp, while in older lenses this same antiserum recognizes mainly the 22000 MW in vivo proteolysis product. In contrast, during aging of the normal human lens anti-βBp1-12 recognizes only decreasing amounts of the 26000 MW form of βBp, with no binding to the 22000 MW form of this polypeptide. These results suggest that during aging of the normal human lens, the N-terminus of βBp is the preferred site of in vivo proteolysis.

Original languageEnglish
Pages (from-to)385-392
Number of pages8
JournalExperimental Eye Research
Volume45
Issue number3
DOIs
Publication statusPublished - 1987

Fingerprint

Crystallins
Lenses
Proteolysis
Immune Sera
Peptides

Keywords

  • human lens
  • lens crystallins
  • βBp

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems

Cite this

Takemoto, L., Takemoto, D., Brown, G., Takehana, M., Smith, J., & Horwitz, J. (1987). Cleavage from the N-terminal region of βBp crystallin during aging of the human lens. Experimental Eye Research, 45(3), 385-392. https://doi.org/10.1016/S0014-4835(87)80125-2

Cleavage from the N-terminal region of βBp crystallin during aging of the human lens. / Takemoto, L.; Takemoto, D.; Brown, G.; Takehana, M.; Smith, J.; Horwitz, J.

In: Experimental Eye Research, Vol. 45, No. 3, 1987, p. 385-392.

Research output: Contribution to journalArticle

Takemoto, L, Takemoto, D, Brown, G, Takehana, M, Smith, J & Horwitz, J 1987, 'Cleavage from the N-terminal region of βBp crystallin during aging of the human lens', Experimental Eye Research, vol. 45, no. 3, pp. 385-392. https://doi.org/10.1016/S0014-4835(87)80125-2
Takemoto, L. ; Takemoto, D. ; Brown, G. ; Takehana, M. ; Smith, J. ; Horwitz, J. / Cleavage from the N-terminal region of βBp crystallin during aging of the human lens. In: Experimental Eye Research. 1987 ; Vol. 45, No. 3. pp. 385-392.
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