Clinical significance of evaluating hormone receptor and HER2 protein using cell block against metastatic breast cancer: A multi-institutional study

Akira Matsui, Yuya Murata, Norikazu Masuda, Kiyoshi Mori, Masato Takahashi, Katsushige Yamashiro, Kenjirou Aogi, Shigeto Maeda, Masahiro Itou, Shinji Ozaki, Kazuya Kuraoka, Yasuyuki Satou, Shu Ichihara, Eriko Tokunaga, Kenichi Taguchi, Takanori Watanabe, Hiroyoshi Suzuki, Aiko Nagayama, Rieko Nishimura

Research output: Contribution to journalArticle

Abstract

Hormone receptor and human epidermal growth factor receptor 2 (HER2) protein tests in metastatic breast cancer tissue are recommended in the guidelines of the American Society of Clinical Oncology/American Pathology Association. As part of a multi-institutional study by the National Hospital Organization, we conducted an investigation to examine these molecular markers, using cytological specimens as a substitute for tissue specimens from breast cancer metastasis. To confirm the usefulness of receptors tested in metastatic lesions, the treatment course of registered metastatic breast cancer patients was analyzed. During the April 2015 to March 2016 registration period, there were 62 registrations. Types of metastatic lesions include pleural fluid (44 samples), ascites (14 samples), lymph nodes (2 samples), pericardial fluid (1 sample), and dorsal subcutaneous mass (1 sample). A stable test result was obtained by adopting the receptor examination method, using cell block for immunostaining cytological specimens. The discordance rates of estrogen receptor (ER), progesterone receptor (PR), and HER2 protein expression were 18.2% (95% confidence interval (CI): 7.9-28.8%), 36.4% (95% CI: 23.7-49.1%), and 8.2% (95% CI: 0.1-16.3%), respectively, between the primary tumor and metastatic lesion. Patients who changed from primary negative to metastatic positive ER status had taken a significantly longer time for metastatic foci to appear. Patients with positive ER status in metastatic lesions had significantly better prognosis than ER-negative cases (P = 0.030) by the Log-Rank test. The ER status of the metastatic lesion and the metastatic site were independent prognostic factors by Cox multivariate analysis. Receptor examination with cytological specimens in metastatic lesions has been useful as it provides guidance for the treatment of metastatic breast cancer.

Original languageEnglish
Pages (from-to)5680-5689
Number of pages10
JournalOncotarget
Volume10
Issue number55
Publication statusPublished - 2019
Externally publishedYes

Keywords

  • Cell block
  • Cytological specimen
  • Metastatic breast cancer
  • Prognosis
  • Receptor discordance

ASJC Scopus subject areas

  • Oncology

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  • Cite this

    Matsui, A., Murata, Y., Masuda, N., Mori, K., Takahashi, M., Yamashiro, K., Aogi, K., Maeda, S., Itou, M., Ozaki, S., Kuraoka, K., Satou, Y., Ichihara, S., Tokunaga, E., Taguchi, K., Watanabe, T., Suzuki, H., Nagayama, A., & Nishimura, R. (2019). Clinical significance of evaluating hormone receptor and HER2 protein using cell block against metastatic breast cancer: A multi-institutional study. Oncotarget, 10(55), 5680-5689.