Clinical significance of telomerase activity in peripheral blood of patients with esophageal squamous cell carcinoma

Kazuo Koyanagi, Soji Ozawa, Nobutoshi Ando, Yuukou Kitagawa, Masakazu Ueda, Masaki Kitajima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background. The presence of tumor cells in the blood stream is considered evidence of a high risk of distant organ metastasis. We examined the usefulness of telomerase activity in peripheral blood polymorphonuclear cells as an indicator of distant metastasis in patients with esophageal squamous cell carcinoma. Methods. Telomerase activity was measured in the peripheral blood mononuclear cell and polymorphonuclear cell fractions obtained from blood samples of healthy volunteers mixed with squamous cell carcinoma cell lines, and cell distribution was analyzed by flow cytometry. Then telomerase activity of forty-two polymorphonuclear cell fractions obtained from esophageal squamous cell carcinoma patients was measured. Results. Telomerase activity was detected in polymorphonuclear cell fractions and cell distribution analysis revealed the presence of esophageal squamous cell carcinoma cells. Organ metastasis was detected in 7 (78%) of the 9 patients with telomerase-positive polymorphonuclear cell fractions as opposed to only five (15%) of the 33 with telomerase-negative cases, and there was a significant positive correlation between telomerase activity and organ metastasis (p < 0.0008). Conclusions. Measurement of telomerase activity in the polymorphonuclear cell fractions is useful for identifying a high risk group for distant organ metastasis in patients with esophageal squamous cell carcinoma.

Original languageEnglish
Pages (from-to)927-932
Number of pages6
JournalAnnals of Thoracic Surgery
Volume73
Issue number3
DOIs
Publication statusPublished - 2002

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Telomerase
Neoplasm Metastasis
Blood Cells
Esophageal Squamous Cell Carcinoma
Squamous Cell Carcinoma
Healthy Volunteers
Flow Cytometry
Cell Line

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Surgery

Cite this

Clinical significance of telomerase activity in peripheral blood of patients with esophageal squamous cell carcinoma. / Koyanagi, Kazuo; Ozawa, Soji; Ando, Nobutoshi; Kitagawa, Yuukou; Ueda, Masakazu; Kitajima, Masaki.

In: Annals of Thoracic Surgery, Vol. 73, No. 3, 2002, p. 927-932.

Research output: Contribution to journalArticle

Koyanagi, Kazuo ; Ozawa, Soji ; Ando, Nobutoshi ; Kitagawa, Yuukou ; Ueda, Masakazu ; Kitajima, Masaki. / Clinical significance of telomerase activity in peripheral blood of patients with esophageal squamous cell carcinoma. In: Annals of Thoracic Surgery. 2002 ; Vol. 73, No. 3. pp. 927-932.
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abstract = "Background. The presence of tumor cells in the blood stream is considered evidence of a high risk of distant organ metastasis. We examined the usefulness of telomerase activity in peripheral blood polymorphonuclear cells as an indicator of distant metastasis in patients with esophageal squamous cell carcinoma. Methods. Telomerase activity was measured in the peripheral blood mononuclear cell and polymorphonuclear cell fractions obtained from blood samples of healthy volunteers mixed with squamous cell carcinoma cell lines, and cell distribution was analyzed by flow cytometry. Then telomerase activity of forty-two polymorphonuclear cell fractions obtained from esophageal squamous cell carcinoma patients was measured. Results. Telomerase activity was detected in polymorphonuclear cell fractions and cell distribution analysis revealed the presence of esophageal squamous cell carcinoma cells. Organ metastasis was detected in 7 (78{\%}) of the 9 patients with telomerase-positive polymorphonuclear cell fractions as opposed to only five (15{\%}) of the 33 with telomerase-negative cases, and there was a significant positive correlation between telomerase activity and organ metastasis (p < 0.0008). Conclusions. Measurement of telomerase activity in the polymorphonuclear cell fractions is useful for identifying a high risk group for distant organ metastasis in patients with esophageal squamous cell carcinoma.",
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AU - Koyanagi, Kazuo

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AU - Ando, Nobutoshi

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AU - Ueda, Masakazu

AU - Kitajima, Masaki

PY - 2002

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N2 - Background. The presence of tumor cells in the blood stream is considered evidence of a high risk of distant organ metastasis. We examined the usefulness of telomerase activity in peripheral blood polymorphonuclear cells as an indicator of distant metastasis in patients with esophageal squamous cell carcinoma. Methods. Telomerase activity was measured in the peripheral blood mononuclear cell and polymorphonuclear cell fractions obtained from blood samples of healthy volunteers mixed with squamous cell carcinoma cell lines, and cell distribution was analyzed by flow cytometry. Then telomerase activity of forty-two polymorphonuclear cell fractions obtained from esophageal squamous cell carcinoma patients was measured. Results. Telomerase activity was detected in polymorphonuclear cell fractions and cell distribution analysis revealed the presence of esophageal squamous cell carcinoma cells. Organ metastasis was detected in 7 (78%) of the 9 patients with telomerase-positive polymorphonuclear cell fractions as opposed to only five (15%) of the 33 with telomerase-negative cases, and there was a significant positive correlation between telomerase activity and organ metastasis (p < 0.0008). Conclusions. Measurement of telomerase activity in the polymorphonuclear cell fractions is useful for identifying a high risk group for distant organ metastasis in patients with esophageal squamous cell carcinoma.

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