Clinical usefulness of chemosensitivity testing using the MTT assay

Toshiharu Furukawa, Tetsuro Kubota, Akihiko Suto, Tetsuya Takahara, Hiroshi Yamaguchi, Tooru Takeuchi, Suguru Kase, Susumu Kodaira, Kyuya Ishibiki, Masaki Kitajima

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

The results of in vitro chemosensitivity testing using the MTT assay of tumor cells from 140 patients were analyzed with reference to the clinical antitumor effects of the chemotherapy. One hundred and twenty‐four (88.6%) of 140 specimens were successfully tested by the method of Mosmann (J Immunol Methods 65:55–63, 1983) with some modifications. When the results of the assay were compared with the clinical effects of chemotherapy in 22 patients with remaining measurable tumor lesions, the overall prediction rate was 86.4% (19/22). Among 31 patients with stage III‐V gastric and colorectal carcinomas without remaining measurable tumor lesions, the survival rate of nine patients treated with drugs shown to be effective in the assay was significantly (P < 0.05) better than that of 22 patients treated with drugs shown to be ineffective.

Original languageEnglish
Pages (from-to)188-193
Number of pages6
JournalJournal of Surgical Oncology
Volume48
Issue number3
DOIs
Publication statusPublished - 1991 Nov

Keywords

  • cancer chemotherapy
  • clinical antitumor effects
  • fresh surgical specimen

ASJC Scopus subject areas

  • Surgery
  • Oncology

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