Clinicopathologic Analysis of Angioimmunoblastic T-cell Lymphoma with or Without RHOA G17V Mutation Using Formalin-fixed Paraffin-embedded Sections

Ryoko Nagao, Yara Yukie Kikuti, Joaquim Carreras, Tomoki Kikuchi, Masashi Miyaoka, Hiromichi Matsushita, Minoru Kojima, Kiyoshi Ando, Mamiko Sakata-Yanagimoto, Shigeru Chiba, Naoya Nakamura

Research output: Contribution to journalArticlepeer-review

24 Citations (Scopus)

Abstract

Angioimmunoblastic T-cell lymphoma (AITL) is an infrequent subtype of peripheral T-cell lymphoma derived from follicular helper T cells. Recently, a somatic G17V RHOA gene mutation has been reported. In this article, we examined the RHOA G17V mutation in 18 cases of AITL by 3 different techniques of Sanger sequencing, fully automated SNP genotyping, and deep sequencing, using routine diagnostic formalin-fixed paraffin-embedded tissue. The RHOA G17V mutation was detected in 10 cases (56%). Among the 10 mutated cases, 8 cases were detected by all 3 methods. The status of RHOA mutation was subsequently compared with the clinicopathologic characteristics of AITL. RHOA-mutated AITL (10 cases) was clinically characterized by high serum IL-2R and a poor ECOG performance status. By immunohistochemistry, expression of CD10, PD-1, CXCL13, and CCR4 and a wide distribution of CD21(+) follicular dendritic cells were observed in RHOA-mutated cases. Among these, CCR4 expression and the CD21(+) network in RHOA-mutated AITL cases were more extensive than in the RHOA mutation-negative AITL cases (P<0.05). Thus, RHOA-mutated AITL cases are more characteristic of follicular helper T cells, and the presence of such a mutation is an important marker for AITL.

Original languageEnglish
Pages (from-to)1041-1050
Number of pages10
JournalAmerican Journal of Surgical Pathology
Volume40
Issue number8
DOIs
Publication statusPublished - 2016 Aug 1
Externally publishedYes

Keywords

  • angioimmunoblastic T-cell lymphoma
  • follicular dendritic cells
  • immunohistochemistry
  • PD-1
  • RHOA mutation

ASJC Scopus subject areas

  • Anatomy
  • Surgery
  • Pathology and Forensic Medicine

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