Clinicopathologic significance of laminin-5 γ2 chain expression in squamous cell carcinoma of the tongue: Immunohistochemical analysis of 67 lesions

Ono Yukiko, Yukihiro Nakanishi, Ino Yoshinori, Toshiro Niki, Tesshi Yamada, Kimio Yoshimura, Masahisa Saikawa, Tamio Nakajima, Setsuo Hirohashi

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

BACKGROUND. The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. METHODS. Laminin-5 γ2 chain expression in squamous cell carcinomas of the tongue in 67 patients with Stage II, III, or IVA,B (excluding the cases with distant metastasis) was examined immunohistochemically to determine its associations with the clinicopathologic features of each tumor. The predominant staining patterns were categorized as follows: A, few or no tumor cells were positive; B, part of the tumor nest periphery was positive; C, the tumor nest periphery was circumferentially positive; or D, almost all the tumor cells were positive. RESULTS. Laminin-5 γ2 chain expression was observed clearly in tumor cell cytoplasm. Of the 67 tumors examined, 6 (9%), 31 (46%), 19 (28%), and 11 (17%) showed staining patterns A, B, C, and D, respectively. With progression from staining pattern A to D, the number of immunopositive tumor cells increased significantly (P < 0.0001), and the tumor histology showed significantly more infiltrative growth (P < 0.0001) and poorer differentiation (P = 0.0021). Furthermore, both univariate (P = 0.0019) and multivariate (P = 0.0003; hazard ratio = 3.132) analysis of the patients' survival revealed that the prognosis became significantly poorer with progression from staining pattern A to D. CONCLUSIONS. Increased laminin-5 γ2 chain immunoreactivity, which may reflect a high invasive potential of cancer cells, is a factor indicative of a poor prognosis for patients with squamous cell carcinoma of the tongue.

Original languageEnglish
Pages (from-to)2315-2321
Number of pages7
JournalCancer
Volume85
Issue number11
DOIs
Publication statusPublished - 1999 Jun 1
Externally publishedYes

Fingerprint

Tongue
Squamous Cell Carcinoma
Neoplasms
Staining and Labeling
kalinin
Survival Analysis
Cell Movement
Histology
Cytoplasm
Neoplasm Metastasis

Keywords

  • Immunohistochemistry
  • Laminin-5 γ2 chain
  • Squamous cell carcinoma
  • Tongue

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Clinicopathologic significance of laminin-5 γ2 chain expression in squamous cell carcinoma of the tongue : Immunohistochemical analysis of 67 lesions. / Yukiko, Ono; Nakanishi, Yukihiro; Yoshinori, Ino; Niki, Toshiro; Yamada, Tesshi; Yoshimura, Kimio; Saikawa, Masahisa; Nakajima, Tamio; Hirohashi, Setsuo.

In: Cancer, Vol. 85, No. 11, 01.06.1999, p. 2315-2321.

Research output: Contribution to journalArticle

Yukiko, Ono ; Nakanishi, Yukihiro ; Yoshinori, Ino ; Niki, Toshiro ; Yamada, Tesshi ; Yoshimura, Kimio ; Saikawa, Masahisa ; Nakajima, Tamio ; Hirohashi, Setsuo. / Clinicopathologic significance of laminin-5 γ2 chain expression in squamous cell carcinoma of the tongue : Immunohistochemical analysis of 67 lesions. In: Cancer. 1999 ; Vol. 85, No. 11. pp. 2315-2321.
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abstract = "BACKGROUND. The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. METHODS. Laminin-5 γ2 chain expression in squamous cell carcinomas of the tongue in 67 patients with Stage II, III, or IVA,B (excluding the cases with distant metastasis) was examined immunohistochemically to determine its associations with the clinicopathologic features of each tumor. The predominant staining patterns were categorized as follows: A, few or no tumor cells were positive; B, part of the tumor nest periphery was positive; C, the tumor nest periphery was circumferentially positive; or D, almost all the tumor cells were positive. RESULTS. Laminin-5 γ2 chain expression was observed clearly in tumor cell cytoplasm. Of the 67 tumors examined, 6 (9{\%}), 31 (46{\%}), 19 (28{\%}), and 11 (17{\%}) showed staining patterns A, B, C, and D, respectively. With progression from staining pattern A to D, the number of immunopositive tumor cells increased significantly (P < 0.0001), and the tumor histology showed significantly more infiltrative growth (P < 0.0001) and poorer differentiation (P = 0.0021). Furthermore, both univariate (P = 0.0019) and multivariate (P = 0.0003; hazard ratio = 3.132) analysis of the patients' survival revealed that the prognosis became significantly poorer with progression from staining pattern A to D. CONCLUSIONS. Increased laminin-5 γ2 chain immunoreactivity, which may reflect a high invasive potential of cancer cells, is a factor indicative of a poor prognosis for patients with squamous cell carcinoma of the tongue.",
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T1 - Clinicopathologic significance of laminin-5 γ2 chain expression in squamous cell carcinoma of the tongue

T2 - Immunohistochemical analysis of 67 lesions

AU - Yukiko, Ono

AU - Nakanishi, Yukihiro

AU - Yoshinori, Ino

AU - Niki, Toshiro

AU - Yamada, Tesshi

AU - Yoshimura, Kimio

AU - Saikawa, Masahisa

AU - Nakajima, Tamio

AU - Hirohashi, Setsuo

PY - 1999/6/1

Y1 - 1999/6/1

N2 - BACKGROUND. The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. METHODS. Laminin-5 γ2 chain expression in squamous cell carcinomas of the tongue in 67 patients with Stage II, III, or IVA,B (excluding the cases with distant metastasis) was examined immunohistochemically to determine its associations with the clinicopathologic features of each tumor. The predominant staining patterns were categorized as follows: A, few or no tumor cells were positive; B, part of the tumor nest periphery was positive; C, the tumor nest periphery was circumferentially positive; or D, almost all the tumor cells were positive. RESULTS. Laminin-5 γ2 chain expression was observed clearly in tumor cell cytoplasm. Of the 67 tumors examined, 6 (9%), 31 (46%), 19 (28%), and 11 (17%) showed staining patterns A, B, C, and D, respectively. With progression from staining pattern A to D, the number of immunopositive tumor cells increased significantly (P < 0.0001), and the tumor histology showed significantly more infiltrative growth (P < 0.0001) and poorer differentiation (P = 0.0021). Furthermore, both univariate (P = 0.0019) and multivariate (P = 0.0003; hazard ratio = 3.132) analysis of the patients' survival revealed that the prognosis became significantly poorer with progression from staining pattern A to D. CONCLUSIONS. Increased laminin-5 γ2 chain immunoreactivity, which may reflect a high invasive potential of cancer cells, is a factor indicative of a poor prognosis for patients with squamous cell carcinoma of the tongue.

AB - BACKGROUND. The laminin-5 γ2 chain plays an important role in cell migration during tumor invasion and tissue remodeling. METHODS. Laminin-5 γ2 chain expression in squamous cell carcinomas of the tongue in 67 patients with Stage II, III, or IVA,B (excluding the cases with distant metastasis) was examined immunohistochemically to determine its associations with the clinicopathologic features of each tumor. The predominant staining patterns were categorized as follows: A, few or no tumor cells were positive; B, part of the tumor nest periphery was positive; C, the tumor nest periphery was circumferentially positive; or D, almost all the tumor cells were positive. RESULTS. Laminin-5 γ2 chain expression was observed clearly in tumor cell cytoplasm. Of the 67 tumors examined, 6 (9%), 31 (46%), 19 (28%), and 11 (17%) showed staining patterns A, B, C, and D, respectively. With progression from staining pattern A to D, the number of immunopositive tumor cells increased significantly (P < 0.0001), and the tumor histology showed significantly more infiltrative growth (P < 0.0001) and poorer differentiation (P = 0.0021). Furthermore, both univariate (P = 0.0019) and multivariate (P = 0.0003; hazard ratio = 3.132) analysis of the patients' survival revealed that the prognosis became significantly poorer with progression from staining pattern A to D. CONCLUSIONS. Increased laminin-5 γ2 chain immunoreactivity, which may reflect a high invasive potential of cancer cells, is a factor indicative of a poor prognosis for patients with squamous cell carcinoma of the tongue.

KW - Immunohistochemistry

KW - Laminin-5 γ2 chain

KW - Squamous cell carcinoma

KW - Tongue

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