Clonal composition of malignant fibrous histiocytoma: Analysis by PCR-based assay of the human androgen receptor gene (HUMARA)

Masaki Q. Fujita, Noriyasu Hashida, Masaru Shin, Hirofumi Nakanishi, Wataru Yoshihara, Katsuyuki Aozasa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Malignant fibrous histiocytoma (MFH), the most common soft-tissue sarcoma, consists mainly of two different cell populations: histiocytelike and fibroblastlike cells. It has been suggested to contain a large amount of reactive histiocytes and fibroblasts hard to distinguish from the tumor cells. In this study, the clonality of MFH was determined by analyzing the patterns of X chromosome inactivation at the human androgen receptor gene (HUMARA)) using DNA samples from archival snap-frozen and paraffin-embedded tissues. All the eleven informative female heterozygotes without severe inflammation showed the monoclonal pattern; 8 storiform-pleomorphic (6 distinct, 2 relative monoclonal pattern) and 3 myxoid (3 distinct monoclonal pattern) subtype. Although normal tissue DNA, amplifiable by the polymerase chain reaction, valid for the assay was not available in these cases, statistically at least 5 cases are monoclonal (p = 0.037 < 0.05), even when markedly skewed lyonization were to primarily exist in the normal tissue at the highest rate as ever reported (33%). Experiments using the mixture of monoclonal and polyclonal DNA at varying ratios have suggested that a distinct monoclonal pattern is obtained only when the monoclonal component exceeds 80%. Our study demonstrates that most cells that are present in MFH are monoclonal in origin which may be the population of tumor cells.

Original languageEnglish
Pages (from-to)600-606
Number of pages7
JournalOncology
Volume55
Issue number6
DOIs
Publication statusPublished - 1998 Nov

Keywords

  • Androgen receptor
  • Clonality analysis, malignant fibrous histiocytoma
  • Malignant fibrous histiocytoma
  • Polymerase chain reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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