Cloning and characterization of a novel gene promoting ureteric bud branching in the metanephros

Takashi Araki, Matsuhiko Hayashi, Takao Saruta

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Background. The ureteric buds and metanephric mesenchymal cells reciprocally induce each other's maturation during kidney development, and implicated transcription factors, secreted growth factors, and cell surface signaling peptides are critical regulators of renal branching morphogenesis. Protein kinase C (PKC) is a key enzyme in the signal transduction mechanisms in various biologic processes, including development, because it regulates growth and differentiation. Inhibition of PKC by the sphingolipid product ceramide interferes with nephron formation in the developing kidney, but the molecule that controls ureteric bud branching downstream of PKC is still unknown. Methods. Differential display polymerase chain reaction (PCR) of metanephroi cultured with a PKC activator and inhibitor was performed. We also examined the role of a novel gene in kidney development with organ culture system. Results. A novel gene encoding a 759 bp mRNA was identified, and we named it metanephros-derived tubulogenic factor (MTF)/L47. Inhibition of MTF with antisense oligonucleotide impaired ureteric bud branching by cultured metanephroi, and addition of recombinant MTF protein promoted ureteric bud branching in cultured metanephroi and increased cell proliferation. Conclusion. We identified a novel molecule in developing kidney that is capable of modulating ureteric bud branching and kidney differentiation.

Original languageEnglish
Pages (from-to)1968-1977
Number of pages10
JournalKidney International
Volume64
Issue number6
DOIs
Publication statusPublished - 2003 Dec

Fingerprint

Organism Cloning
Kidney
Protein Kinase C
Genes
Sphingolipids
Protein C Inhibitor
Antisense Oligonucleotides
Ceramides
Organ Culture Techniques
Nephrons
Protein Kinase Inhibitors
Morphogenesis
Signal Transduction
Intercellular Signaling Peptides and Proteins
Transcription Factors
Cell Proliferation
Polymerase Chain Reaction
Messenger RNA
Peptides
Enzymes

Keywords

  • Cell proliferation
  • Metanephros
  • PKC
  • Ureteric bud branching

ASJC Scopus subject areas

  • Nephrology

Cite this

Cloning and characterization of a novel gene promoting ureteric bud branching in the metanephros. / Araki, Takashi; Hayashi, Matsuhiko; Saruta, Takao.

In: Kidney International, Vol. 64, No. 6, 12.2003, p. 1968-1977.

Research output: Contribution to journalArticle

Araki, Takashi ; Hayashi, Matsuhiko ; Saruta, Takao. / Cloning and characterization of a novel gene promoting ureteric bud branching in the metanephros. In: Kidney International. 2003 ; Vol. 64, No. 6. pp. 1968-1977.
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