Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger protein, Ciz1

Kaoru Mitsui, Akira Matsumoto, Satoshi Ohtsuka, Motoaki Ohtsubo, Akihiko Yoshimura

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

p21(Cip1/Waf1) inhibits cell-cycle progression by binding to G1 cyclin/CDK complexes and proliferating cell nuclear antigen (PCNA) through its N- and C-terminal domains, respectively. Here, we report a novel p21(Cip1/Waf1)-interacting protein, Ciz1 (for Cip1 interacting zinc finger protein), which contains polyglutamine repeats and glutamine-rich region in the N-terminus as well as three zinc-finger motifs and one MH3 (matrin 3-homologous domain 3) in the C-terminal region, Ciz1 bound to the N-terminal, the CDK2-interacting part of p21(Cip1/Waf1), and the interaction was disrupted by the overexpression of CDK2. A region of about 150 amino acids containing the first zinc-finger motif in Ciz1 was the binding site for p21(Cip/Waf1). When Ciz1 and p21(Cip/Waf1) were individually overexpressed in U2-OS cells, they mostly localized in the nucleus. However, coexpression of Ciz1 induced cytoplasmic distribution of p21(Cip1/Waf1). These data indicate that Ciz1 is a unique nuclear protein that regulates the cellular localization of p21(Cip/Waf1).

Original languageEnglish
Pages (from-to)457-464
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume264
Issue number2
DOIs
Publication statusPublished - 1999 Oct 22
Externally publishedYes

Fingerprint

Cloning
Zinc Fingers
Zinc
Organism Cloning
Cyclin G1
Proteins
Proliferating Cell Nuclear Antigen
Nuclear Proteins
Glutamine
Cell Cycle
Binding Sites
Cells
Amino Acids

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger protein, Ciz1. / Mitsui, Kaoru; Matsumoto, Akira; Ohtsuka, Satoshi; Ohtsubo, Motoaki; Yoshimura, Akihiko.

In: Biochemical and Biophysical Research Communications, Vol. 264, No. 2, 22.10.1999, p. 457-464.

Research output: Contribution to journalArticle

Mitsui, Kaoru ; Matsumoto, Akira ; Ohtsuka, Satoshi ; Ohtsubo, Motoaki ; Yoshimura, Akihiko. / Cloning and characterization of a novel p21(Cip1/Waf1)-interacting zinc finger protein, Ciz1. In: Biochemical and Biophysical Research Communications. 1999 ; Vol. 264, No. 2. pp. 457-464.
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