Cloning and characterization of DIP1, a novel protein that is related to the id family of proteins

Yao Yao, Yuichiro Doki, Wei Jiang, Masaya Imoto, V. S. Venkatraj, Dorothy Warburton, Regina M. Santella, Binfeng Lu, Lunbiao Yan, Xiao Hong Sun, Tao Su, Jingqing Luo, I. Bernard Weinstein

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Using human cyclin D1 as the 'bait' in a yeast two-hybrid system, together with a HL60 cDNA library, we identified a novel human nuclear protein designated DIP1. This protein is expressed in a variety of cell types, and in fibroblasts its level remains constant throughout the cell cycle. However, the level of this protein increases severalfold during the differentiation of HL60 cells. The DIP1 protein can be phosphorylated in vitro by a cellular kinase and this activity reaches its maximum in extracts obtained from cells in the G1 phase of the cell cycle. DIP1 contains a helix- loop-helix motif but lacks an adjacent basic DNA-binding domain, thus resembling the Id family of proteins. The dip1 gene is located on human chromosome 16pl 1.2-12, a locus that is amplified in several types of human cancer. These results suggest that DIP1 may be involved in the control of gene expression and differentiation, but its precise function remains to be determined. (C) 2000 Academic Press.

Original languageEnglish
Pages (from-to)22-32
Number of pages11
JournalExperimental Cell Research
Volume257
Issue number1
DOIs
Publication statusPublished - 2000 May 25
Externally publishedYes

Fingerprint

Organism Cloning
Cell Cycle
Helix-Loop-Helix Motifs
Proteins
Two-Hybrid System Techniques
HL-60 Cells
G1 Phase
Human Chromosomes
Nuclear Proteins
Cell Extracts
Gene Library
Phosphotransferases
Fibroblasts
Gene Expression
DNA
Genes
Neoplasms

Keywords

  • Cyclin D1
  • Differentiation
  • DIP1
  • HL60
  • Id

ASJC Scopus subject areas

  • Cell Biology

Cite this

Cloning and characterization of DIP1, a novel protein that is related to the id family of proteins. / Yao, Yao; Doki, Yuichiro; Jiang, Wei; Imoto, Masaya; Venkatraj, V. S.; Warburton, Dorothy; Santella, Regina M.; Lu, Binfeng; Yan, Lunbiao; Sun, Xiao Hong; Su, Tao; Luo, Jingqing; Weinstein, I. Bernard.

In: Experimental Cell Research, Vol. 257, No. 1, 25.05.2000, p. 22-32.

Research output: Contribution to journalArticle

Yao, Y, Doki, Y, Jiang, W, Imoto, M, Venkatraj, VS, Warburton, D, Santella, RM, Lu, B, Yan, L, Sun, XH, Su, T, Luo, J & Weinstein, IB 2000, 'Cloning and characterization of DIP1, a novel protein that is related to the id family of proteins', Experimental Cell Research, vol. 257, no. 1, pp. 22-32. https://doi.org/10.1006/excr.2000.4884
Yao, Yao ; Doki, Yuichiro ; Jiang, Wei ; Imoto, Masaya ; Venkatraj, V. S. ; Warburton, Dorothy ; Santella, Regina M. ; Lu, Binfeng ; Yan, Lunbiao ; Sun, Xiao Hong ; Su, Tao ; Luo, Jingqing ; Weinstein, I. Bernard. / Cloning and characterization of DIP1, a novel protein that is related to the id family of proteins. In: Experimental Cell Research. 2000 ; Vol. 257, No. 1. pp. 22-32.
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