Cloning and characterization of NE-dlg: A novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein interacts with the APC protein

Keishi Makino, Hiroaki Kuwahara, Norio Masuko, Yasuyuki Nishiyama, Tetsurou Morisaki, Ji Ichiro Sasaki, Mitsuyoshi Nakao, Akira Kuwano, Motomi Nakata, Yukitaka Ushio, Hideyuki Saya

Research output: Contribution to journalArticlepeer-review

55 Citations (Scopus)

Abstract

We have cloned a cDNA for a novel human homolog of the Drosophila discs large (dlg) tumor suppressor protein, termed NE-dlg (neuronal and endocrine dlg). Northern blot analysis revealed that the gene is highly expressed in neuronal and endocrine tissues. Fluorescence in situ hybridization (FISH) and radiation hybrid mapping studies localized the NE-dlg gene to chromosome Xq13. We also found that the NE-dlg gene encoded a 100 kDa protein. Immunolocalization studies using an NE-dlg antibody showed that the protein tended to be expressed in non-proliferating cells, such as neurons, cells in Langerhans islets of the pancreas, myocytes of the heart muscles, and the prickle and functional layer cells of the esophageal epithelium. Proliferative cells, including various cultured cancer cell lines and basal cells in the esophageal epithelium, showed little expression of the NE-dlg protein. In addition, yeast two-hybrid screening and in vitro binding assays revealed that the NE-dlg interacted with the carboxyl-terminal region of the APC tumor suppressor protein. These data suggest that NE-dlg negatively regulates cell proliferation through its interaction with the APC protein.

Original languageEnglish
Pages (from-to)2425-2433
Number of pages9
JournalOncogene
Volume14
Issue number20
DOIs
Publication statusPublished - 1997 Jul 8
Externally publishedYes

Keywords

  • Chromosome Xq13
  • EST
  • Tumor suppressor genes
  • Two-hybrid screening

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

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