Cloning and characterization of Xenopus laevis xSox7 cDNA

Masahide Shiozawa, Yoshiki Hiraoka, Naoki Komatsu, Motoyuki Ogawa, Yukinao Sakai, Sadakazu Aiso

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

A family of SRY-related genes has been termed SOX. We have isolated and sequenced a cDNA encoding a novel Sox protein from Xenopus laevis ovary. This cDNA contains an open reading frame (ORF) coding for 362 amino acids, which encompasses an HMG box and exhibits a strong (90%) identity to that of mouse Sox7; the cDNA was named xSox7 in this study. Northern analysis revealed that the xSox7 mRNA was 2.0 kb in length. Various adult frog tissues were tested by reverse transcription/polymerase chain reaction for xSox7 mRNA, and the results showed that xSox7 is expressed in a wide range of tissues. Furthermore, electrophoretic mobility shift assay indicated that recombinant xSox7 is capable of binding to AACAAT sequence.

Original languageEnglish
Pages (from-to)73-76
Number of pages4
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1309
Issue number1-2
DOIs
Publication statusPublished - 1996 Nov 11

Fingerprint

Cloning
Xenopus laevis
Organism Cloning
Complementary DNA
Xenopus Proteins
Tissue
Electrophoretic mobility
Messenger RNA
Polymerase chain reaction
Electrophoretic Mobility Shift Assay
Transcription
Anura
Open Reading Frames
Reverse Transcription
Ovary
Assays
Genes
Amino Acids
Polymerase Chain Reaction

Keywords

  • (Xenopus laevis)
  • cDNA
  • Nucleotide sequence
  • SOX
  • SRY

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Structural Biology
  • Biophysics

Cite this

Cloning and characterization of Xenopus laevis xSox7 cDNA. / Shiozawa, Masahide; Hiraoka, Yoshiki; Komatsu, Naoki; Ogawa, Motoyuki; Sakai, Yukinao; Aiso, Sadakazu.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1309, No. 1-2, 11.11.1996, p. 73-76.

Research output: Contribution to journalArticle

Shiozawa, Masahide ; Hiraoka, Yoshiki ; Komatsu, Naoki ; Ogawa, Motoyuki ; Sakai, Yukinao ; Aiso, Sadakazu. / Cloning and characterization of Xenopus laevis xSox7 cDNA. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1996 ; Vol. 1309, No. 1-2. pp. 73-76.
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