Cloning and expression of a protein kinase C-regulated chloride channel abundantly expressed in rat brain neuronal cells

Masanobu Kawasaki, Shinichi Uchida, Toshiaki Monkawa, Atsushi Miyawaki, Katsuhiko Mikoshiba, Fumiaki Marumo, Sei Sasaki

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Abstract

cDNA (CIC-3) encoding a protein kinase C-regulated chloride channel was cloned and characterized. The open reading frame encodes 760 amino acids, which possess significant amino acid identity with previously cloned CIC chloride channels. The chloride currents expressed in Xenopus oocytes injected with CIC-3 cRNA were completely blocked by activation of protein kinase C by 12-O-tetradecanoylphorbol 13-acetate. Abundant expression of CIC-3 mRNA was observed in rat brain, especially in the olfactory bulb, hippocampus, and cerebellum. These findings suggest that CIC-3 may play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C.

Original languageEnglish
Pages (from-to)597-604
Number of pages8
JournalNeuron
Volume12
Issue number3
DOIs
Publication statusPublished - 1994 Mar

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ASJC Scopus subject areas

  • Neuroscience(all)

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