Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter

Wakano Ogawa, Young Mog Kim, Tohru Mizushima, Tomofusa Tsuchiya

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We cloned a gene (sstT) for the Na+/serine symporter from the chromosome of Escherichia coli by using a low-copy-number vector and sequenced it. According to the deduced amino acid sequence, the transporter (SstT) consists of 414 amino acid residues. Hydropathy analysis suggested that the SstT protein possesses 9, instead of 12, hydrophobic domains.

Original languageEnglish
Pages (from-to)6749-6752
Number of pages4
JournalJournal of Bacteriology
Volume180
Issue number24
Publication statusPublished - 1998 Dec
Externally publishedYes

Fingerprint

Symporters
Amino Acid Transport Systems
Serine
Organism Cloning
Amino Acid Sequence
Chromosomes
Escherichia coli
Gene Expression
Amino Acids
Genes
Proteins

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter. / Ogawa, Wakano; Kim, Young Mog; Mizushima, Tohru; Tsuchiya, Tomofusa.

In: Journal of Bacteriology, Vol. 180, No. 24, 12.1998, p. 6749-6752.

Research output: Contribution to journalArticle

Ogawa, Wakano ; Kim, Young Mog ; Mizushima, Tohru ; Tsuchiya, Tomofusa. / Cloning and expression of the gene for the Na+-coupled serine transporter from Escherichia coli and characteristics of the transporter. In: Journal of Bacteriology. 1998 ; Vol. 180, No. 24. pp. 6749-6752.
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