Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver

Shigeru Ko, Shinichi Uchida, Satoru Naruse, Michio Kuwahara, Kenichi Ishibashi, Fumiaki Marumo, Tetsuo Hayakawa, Sei Sasaki

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

A new aquaporin was isolated from rat liver based on homology to known aquaporins. A 1408 bp cDNA was sequenced (designated rAQP9L) with a 885 bp open reading frame encoding a 295 amino acid hydrophobic protein. rAQP9L has the greatest amino-acid sequence identity with human AQP9 (75%) and a less homology with AQP3 (49%) and AQP7 (47%). Northern blot analysis indicated a 1.4-kb transcript expressed strongly in liver>testis>brain=lung. Expression of rAQP9L cRNA in Xenopus oocytes increased osmotic water permeability by 6-folds which was inhibited by 0.3 mM mercury chloride by 42%. rAQP9L also facilitated glycerol and urea transport by 2- and 5-folds, respectively. The large discrepancy of tissue distribution between hAQP9 and rAQP9L suggest that rAQP9L is a new aquaporin, which is involved in transport of urea as well as water in liver.

Original languageEnglish
Pages (from-to)309-318
Number of pages10
JournalBiochemistry and Molecular Biology International
Volume47
Issue number2
Publication statusPublished - 1999 Feb
Externally publishedYes

Fingerprint

Aquaporins
Cloning
Liver
Rats
Organism Cloning
Urea
Amino Acids
Complementary RNA
Water
Tissue Distribution
Xenopus
Mercury
Northern Blotting
Glycerol
Open Reading Frames
Oocytes
Testis
Chlorides
Amino Acid Sequence
Permeability

Keywords

  • Aquaporin
  • Glycerol
  • Rat liver
  • Urea

ASJC Scopus subject areas

  • Biochemistry
  • Genetics
  • Molecular Biology

Cite this

Ko, S., Uchida, S., Naruse, S., Kuwahara, M., Ishibashi, K., Marumo, F., ... Sasaki, S. (1999). Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver. Biochemistry and Molecular Biology International, 47(2), 309-318.

Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver. / Ko, Shigeru; Uchida, Shinichi; Naruse, Satoru; Kuwahara, Michio; Ishibashi, Kenichi; Marumo, Fumiaki; Hayakawa, Tetsuo; Sasaki, Sei.

In: Biochemistry and Molecular Biology International, Vol. 47, No. 2, 02.1999, p. 309-318.

Research output: Contribution to journalArticle

Ko, S, Uchida, S, Naruse, S, Kuwahara, M, Ishibashi, K, Marumo, F, Hayakawa, T & Sasaki, S 1999, 'Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver', Biochemistry and Molecular Biology International, vol. 47, no. 2, pp. 309-318.
Ko, Shigeru ; Uchida, Shinichi ; Naruse, Satoru ; Kuwahara, Michio ; Ishibashi, Kenichi ; Marumo, Fumiaki ; Hayakawa, Tetsuo ; Sasaki, Sei. / Cloning and functional expression of rAQP9L a new member of aquaporin family from rat liver. In: Biochemistry and Molecular Biology International. 1999 ; Vol. 47, No. 2. pp. 309-318.
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