Abstract
A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.
| Original language | English |
|---|---|
| Pages (from-to) | 43-49 |
| Number of pages | 7 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 166 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 1990 Jan 15 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Cloning of a hsp70-related gene expressed in mouse spermatids. / Matsumoto, Midori; Fujimoto, Hirokazu.
In: Biochemical and Biophysical Research Communications, Vol. 166, No. 1, 15.01.1990, p. 43-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning of a hsp70-related gene expressed in mouse spermatids
AU - Matsumoto, Midori
AU - Fujimoto, Hirokazu
PY - 1990/1/15
Y1 - 1990/1/15
N2 - A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.
AB - A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.
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UR - http://www.scopus.com/inward/citedby.url?scp=0025058935&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91909-C
DO - 10.1016/0006-291X(90)91909-C
M3 - Article
C2 - 2302214
AN - SCOPUS:0025058935
VL - 166
SP - 43
EP - 49
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -