Cloning of a hsp70-related gene expressed in mouse spermatids

Midori Matsumoto, Hirokazu Fujimoto

Research output: Contribution to journalArticle

105 Citations (Scopus)

Abstract

A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.

Original languageEnglish
Pages (from-to)43-49
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume166
Issue number1
DOIs
Publication statusPublished - 1990 Jan 15
Externally publishedYes

Fingerprint

Spermatids
Cloning
HSP70 Heat-Shock Proteins
Organism Cloning
Genes
Clone Cells
Germ Cells
Cells
Genomic Library
Haploidy
Heat-Shock Proteins
Gene Library
Open Reading Frames
Proteins
Complementary DNA
Tissue
Amino Acids
DNA

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning of a hsp70-related gene expressed in mouse spermatids. / Matsumoto, Midori; Fujimoto, Hirokazu.

In: Biochemical and Biophysical Research Communications, Vol. 166, No. 1, 15.01.1990, p. 43-49.

Research output: Contribution to journalArticle

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