Abstract
A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.
Original language | English |
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Pages (from-to) | 43-49 |
Number of pages | 7 |
Journal | Biochemical and Biophysical Research Communications |
Volume | 166 |
Issue number | 1 |
DOIs | |
Publication status | Published - 1990 Jan 15 |
Externally published | Yes |
ASJC Scopus subject areas
- Biophysics
- Biochemistry
- Molecular Biology
- Cell Biology