Cloning of a hsp70-related gene expressed in mouse spermatids

Midori Matsumoto; Hirokazu Fujimoto

doi:10.1016/0006-291X(90)91909-C

Cloning of a hsp70-related gene expressed in mouse spermatids

Midori Matsumoto, Hirokazu Fujimoto

Research output: Contribution to journal › Article › peer-review

106 Citations (Scopus)

Abstract

A cDNA library of spermatids was screened by a differential hybridization in order to isolate genes expressed in haploid cells of the mouse male germ line. A clone was found that encoded a protein related to the heat shock protein 70. A genomic DNA clone comparable to this cDNA clone was also isolated from a mouse genomic library. This gene had only one continuous open reading frame capable of coding a 630 amino-acid protein. There was an excellent match of the sequence with the heat shock protein 70 family but a difference from any previous 70 kilodalton heat shock protein. A 2.7kb transcript derived from this gene was expressed in spermatids but not in other testicular germ cells and somatic tissues. We have referred to this gene as hsc70t.

Original language	English
Pages (from-to)	43-49
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	166
Issue number	1
DOIs	https://doi.org/10.1016/0006-291X(90)91909-C
Publication status	Published - 1990 Jan 15
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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