TY - JOUR
T1 - Cloning of hexamethylene-bis-acetamide-inducible transcript, HEXIM1, in human vascular smooth muscle cells
AU - Kusuhara, Masatoshi
AU - Nagasaki, Koichi
AU - Kimura, Kensuke
AU - Maass, Nicolai
AU - Manabe, Tomohiro
AU - Ishikawa, Shiro
AU - Aikawa, Minoru
AU - Miyazaki, Koji
AU - Yamaguchi, Ken
PY - 1999
Y1 - 1999
N2 - The dedifferentiation and the abnormal growth of vascular smooth muscle cells (VSMC) is a major component of vascular diseases. Hexamethylene-bis-acetamide (HMBA) has recently been shown to modulate the differentiation and growth of VSMC in vitro and in vivo. To determine the mechanism of this effect, we treated human coronary artery VSMC with HMBA and analyzed the effect of HMBA on gene expression by mRNA differential display. HMBA modulated the expression of a number of specific message fragments. By Marathon extension and cDNA screening, we isolated a novel gene, HEXIM1, that is up-regulated in HMBA-stimulated VSMC. HEXIM1 cDNA has a length of 3624 bp and encodes for a protein of 359 amino acids. Northern blot analysis demonstrated that the level of HEXIM1 mRNA rapidly increased at 1 h after HMBA stimulation and was sustained for 48 h. These results suggest that HEXIM1 plays an important role in the regulation of the growth and differentiation of VSMC.
AB - The dedifferentiation and the abnormal growth of vascular smooth muscle cells (VSMC) is a major component of vascular diseases. Hexamethylene-bis-acetamide (HMBA) has recently been shown to modulate the differentiation and growth of VSMC in vitro and in vivo. To determine the mechanism of this effect, we treated human coronary artery VSMC with HMBA and analyzed the effect of HMBA on gene expression by mRNA differential display. HMBA modulated the expression of a number of specific message fragments. By Marathon extension and cDNA screening, we isolated a novel gene, HEXIM1, that is up-regulated in HMBA-stimulated VSMC. HEXIM1 cDNA has a length of 3624 bp and encodes for a protein of 359 amino acids. Northern blot analysis demonstrated that the level of HEXIM1 mRNA rapidly increased at 1 h after HMBA stimulation and was sustained for 48 h. These results suggest that HEXIM1 plays an important role in the regulation of the growth and differentiation of VSMC.
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U2 - 10.2220/biomedres.20.273
DO - 10.2220/biomedres.20.273
M3 - Article
AN - SCOPUS:0033501002
SN - 0388-6107
VL - 20
SP - 273
EP - 279
JO - Biomedical Research (Japan)
JF - Biomedical Research (Japan)
IS - 5
ER -
