Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA

Akira Shinohara, Hideyuki Ogawa, Yoichi Matsuda, Noriko Kodani, Kazuho Ikeo, Tomoko Ogawa

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double-strand breaks of DNA. We have cloned genes from human, mouse and fission yeast that are homologous to rad51. The 339 amino acid proteins predicted for the two mammalian genes are almost identical and are highly homologous (83%) with the yeast proteins. The mouse gene is transcribed at a high level in thymus, spleen, testis andpvary and at a lower level in brain and other tissues. The rad51 homologues fail to complement the DNA repair defect of rad51 mutants of S. cerevisiae. The mouse gene is located in the F1 region of chromosome 2 and the human gene maps to chromosome 15.

Original languageEnglish
Pages (from-to)239-243
Number of pages5
JournalNature genetics
Volume4
Issue number3
DOIs
Publication statusPublished - 1993 Jan 1
Externally publishedYes

Fingerprint

Schizosaccharomyces
Genetic Recombination
Organism Cloning
Genes
Saccharomyces cerevisiae
Recombinational DNA Repair
Chromosomes, Human, Pair 15
Fungal Proteins
Chromosomes, Human, Pair 2
Double-Stranded DNA Breaks
DNA Repair
Thymus Gland
Testis
Spleen
Escherichia coli
Amino Acids
Brain
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. / Shinohara, Akira; Ogawa, Hideyuki; Matsuda, Yoichi; Kodani, Noriko; Ikeo, Kazuho; Ogawa, Tomoko.

In: Nature genetics, Vol. 4, No. 3, 01.01.1993, p. 239-243.

Research output: Contribution to journalArticle

Shinohara, Akira ; Ogawa, Hideyuki ; Matsuda, Yoichi ; Kodani, Noriko ; Ikeo, Kazuho ; Ogawa, Tomoko. / Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA. In: Nature genetics. 1993 ; Vol. 4, No. 3. pp. 239-243.
@article{c0aa682961e248cfa5db116f890d7e60,
title = "Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA",
abstract = "Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double-strand breaks of DNA. We have cloned genes from human, mouse and fission yeast that are homologous to rad51. The 339 amino acid proteins predicted for the two mammalian genes are almost identical and are highly homologous (83{\%}) with the yeast proteins. The mouse gene is transcribed at a high level in thymus, spleen, testis andpvary and at a lower level in brain and other tissues. The rad51 homologues fail to complement the DNA repair defect of rad51 mutants of S. cerevisiae. The mouse gene is located in the F1 region of chromosome 2 and the human gene maps to chromosome 15.",
author = "Akira Shinohara and Hideyuki Ogawa and Yoichi Matsuda and Noriko Kodani and Kazuho Ikeo and Tomoko Ogawa",
year = "1993",
month = "1",
day = "1",
doi = "10.1038/ng0793-239",
language = "English",
volume = "4",
pages = "239--243",
journal = "Nature Genetics",
issn = "1061-4036",
publisher = "Nature Publishing Group",
number = "3",

}

TY - JOUR

T1 - Cloning of human, mouse and fission yeast recombination genes homologous to RAD51 and recA

AU - Shinohara, Akira

AU - Ogawa, Hideyuki

AU - Matsuda, Yoichi

AU - Kodani, Noriko

AU - Ikeo, Kazuho

AU - Ogawa, Tomoko

PY - 1993/1/1

Y1 - 1993/1/1

N2 - Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double-strand breaks of DNA. We have cloned genes from human, mouse and fission yeast that are homologous to rad51. The 339 amino acid proteins predicted for the two mammalian genes are almost identical and are highly homologous (83%) with the yeast proteins. The mouse gene is transcribed at a high level in thymus, spleen, testis andpvary and at a lower level in brain and other tissues. The rad51 homologues fail to complement the DNA repair defect of rad51 mutants of S. cerevisiae. The mouse gene is located in the F1 region of chromosome 2 and the human gene maps to chromosome 15.

AB - Rad51, of Saccharomyces cerevisiae, is a homologue of recA of Escherichia coli and plays crucial roles in both mitotic and meiotic recombination and in repair of double-strand breaks of DNA. We have cloned genes from human, mouse and fission yeast that are homologous to rad51. The 339 amino acid proteins predicted for the two mammalian genes are almost identical and are highly homologous (83%) with the yeast proteins. The mouse gene is transcribed at a high level in thymus, spleen, testis andpvary and at a lower level in brain and other tissues. The rad51 homologues fail to complement the DNA repair defect of rad51 mutants of S. cerevisiae. The mouse gene is located in the F1 region of chromosome 2 and the human gene maps to chromosome 15.

UR - http://www.scopus.com/inward/record.url?scp=84970062267&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84970062267&partnerID=8YFLogxK

U2 - 10.1038/ng0793-239

DO - 10.1038/ng0793-239

M3 - Article

VL - 4

SP - 239

EP - 243

JO - Nature Genetics

JF - Nature Genetics

SN - 1061-4036

IS - 3

ER -