Cloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto ρ11 temperate bacteriophage

T. Ikeuchi, Jun Kudo, K. Kurahashi

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

A HindIII fragment harboring the intact spo0C gene of Bacillus subtilis was cloned with ρ11 temperate bacteriophage as a vector. Transformation experiments with the DNA from ρ11 dspo0C+ specialized transducing phage showed that the spo0C gene resides on a 5.3-megadalton fragment generated by HindIII digestion. The 5.3-megadalton fragment also contains the intact spo0A gene, but not spoIIIA, spoIIIB, or spoIVB.

Original languageEnglish
Pages (from-to)988-991
Number of pages4
JournalJournal of Bacteriology
Volume154
Issue number2
Publication statusPublished - 1983
Externally publishedYes

Fingerprint

Bacillus subtilis
Bacteriophages
Organism Cloning
Genes
Digestion
DNA

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology
  • Immunology

Cite this

Cloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto ρ11 temperate bacteriophage. / Ikeuchi, T.; Kudo, Jun; Kurahashi, K.

In: Journal of Bacteriology, Vol. 154, No. 2, 1983, p. 988-991.

Research output: Contribution to journalArticle

Ikeuchi, T. ; Kudo, Jun ; Kurahashi, K. / Cloning of sporulation genes spo0A and spo0C of Bacillus subtilis onto ρ11 temperate bacteriophage. In: Journal of Bacteriology. 1983 ; Vol. 154, No. 2. pp. 988-991.
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