Cloning of the gene coding for a shared human melanoma antigen recognized by autologous T cells infiltrating into tumor

Yutaka Kawakami, Siona Eliyahu, Cynthia H. Delgado, Paul F. Robbins, Licia Rivoltini, Suzanne L. Topalian, Toru Miki, Steven A. Rosenberg

Research output: Contribution to journalArticle

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Abstract

By cDNA expression cloning we have isolated a gene encoding a shared human melanoma antigen recognized by HLA-A2 restricted autologous and allogeneic tumor-infiltrating lymphocytes (TILs) from patients with metastatic melanoma. By using both transient and stable expression systems, transfection of this gene into non-antigen-expressing HLA-A2+ cell lines resulted in recognition by the antigen-specific TILs. The sequence of this cDNA revealed a previously undescribed putative transmembrane protein whose expression was restricted to melanoma and melanocyte cell lines and human retina but no other fresh or cultured normal tissues tested or other tumor histologies. Thus, we have identified a gene encoding a melanocyte lineage-specific protein (MART-1; melanoma antigen recognized by T cells 1) that is a widely shared melanoma antigen recognized by the T lymphocytes of patients with established malignancy. Identification of this gene opens possibilities for the development of immunotherapies for patients with melanoma.

Original languageEnglish
Pages (from-to)3515-3519
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume91
Issue number9
DOIs
Publication statusPublished - 1994 Apr 26

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Keywords

  • HLA-A2
  • MART-1
  • immunotherapy
  • melanocyte
  • tumor antigen

ASJC Scopus subject areas

  • General

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