Cloning of the Human α-Catenin cDNA and Its Aberrant mRNA in a Human Cancer Cell Line

Tatsuya Oda, Yae Kanai, Yutaka Shimoyama, Akira Nagafuchi, Shoichiro Tsukita, Setsuo Hirohashi

Research output: Contribution to journalArticle

85 Citations (Scopus)

Abstract

Cadherin and catenin compose cell adhesion complex and are indispensable for tight cell-cell adhesion. Dysfunction of this adhesion complex causes dissociation of cancer cells from primary tumor nodules, thus possibly contributing to cancer invasion and metastasis. In this report, we present the human α-caienin sequence. Human α-catenin showed extensive homology with that of mouse, i.e., 91.8% and 99.3% at the nucleic acid and amino acid levels, respectively, indicating that this molecule has been evolutionarily conserved in mammals. Characterization of the mRNA sequence of α-catenin in PC9 was also carried out, and two distinct abnormal sequences, i.e., one of 957 bp deletion resulting in a 319-amino-acid deletion and another of 761 bp deletion resulting in a frameshift, were identified. These deletions were probably produced by an error of RNA splicing, presenting one possible mechanism for the loss of intact α-catenin expression.

Original languageEnglish
Pages (from-to)897-904
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume193
Issue number3
DOIs
Publication statusPublished - 1993 Jun 30
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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