Cloning of TPA-inducible early (TIE) genes by differential hybridization using TPA-Nonresponsive variant of mouse 3T3-L1 cells

Masayuki Amagai, Yoshio Inokuchi, Takeji Nishikawa, Yoshiko Shimizu, Nobuyoshi Shimizu

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2 Citations (Scopus)


The tumor promoter 12-O -tetradecanoylphorbol-13-acetate (TPA) induces DNA synthesis in quiescent 3T3-L1 cells but not in its variant VT-1 cells. A λgt10 cDNA library was constructed using poly(A)+ RNA from 3T3-L1 cells that were stimulated by TPA for 20 min. Radioactive cDNA probes were prepared from mRNAs of TPA-treated 3 T3-L1 and VT-1 cells and used for screening of the 3T3-L1 cDNA library by differential hybridization. Nine of 6000 phage plaques hybridized only to the 3T3-L1 cDNA probe. Analysis of the nucleotide sequence of five of these clones indicated a high degree of homology with human or mouse type I and type III collagen genes. Three other independent clones showed no homology with any known DNA sequences. These isolated clones of TPA-inducible early (TIE) genes may be useful to study the signal transduction pathway of phorbol esters.

Original languageEnglish
Pages (from-to)153-158
Number of pages6
JournalSomatic Cell and Molecular Genetics
Issue number2
Publication statusPublished - 1989 Mar 1


ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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